Antibody purification is advisable for certain applications, e.g. immunoprecipitation, immunohistochemistry and antibody labeling. Agrisera offers antibody purification of a range of different immunoglobulins:
Please note that:
To obtain antigen-specific antibodies (from serum or IgY fraction), the following steps are performed:
Theoretical yield from affinity purification is around 0.5-5 mg of antigen-specific antibodies per 1 mg of peptide coupled to the support, or 0.5-2 mg of antigen-specific antibodies per 1 mg of protein coupled to the support.
When necessary, antibodies will be carefully eluted in two different steps, including elution at neutral pH, to prevent irreversible denaturation of purified antibodies.
To obtain total immunoglobulin fraction, the following steps are performed:
In case of very low amounts of protein are available for affinity purification, it can be done using this approach.
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