PsbI | Small subunit I of PSII (cyanobacterial)

Product no: AS10 692

AS10 692  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: cyanobacteria

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Synechocystis sp. PCC 6803 PsbI sequence UniProt: Q54697

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 200 l
    Reconstitution: For reconstitution add 200 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 5000-1 : 10 000 (WB)
    Expected | apparent MW:

    4.3 kDa

  • Reactivity
  • Confirmed reactivity: Synechocystis sp. PCC 6803
    Predicted reactivity: Cyanobacteria
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Application example

     

    western blot detection using anti-PsbI antibodies (cyanobacterial)

    Membrane fraction (0.5 µg chl), prepared from Synechocystis sp. PCC 6803, was separated on 12% SDS-PAGE and blotted in 2 hours at RT to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS, probed with anti-PsbI (1:10 000) antibody in TTBS overnight at 8ºC and secondary anti-rabbit in TTBS (1:10000,1h, room temperature). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000.

    Courtesy Dr. Roman Sobotka, Laboratory of Photosynthesis, Trebon, Czech Republic
  • Additional Information
  • Additional information (application): Loads higher than 0,5 g of chlorophyll per well are not recommended
  • Background
  • Background:

    The PsbI protein, previously named the 4.8-kDa protein, is encoded by the plastome. PsbI is a universal component of PSII and is highly conserved (e.g. there is 71% amino acid identicality between the Arabidopsis and Synechocystis 6803 proteins). The protein contains 36 to 38 amino acids in most species, with molecular masses ranging between 4.1 and 4.5 kDa. Synonymes: PSII-I, PSII 4.8 kDa protein

  • Product Citations
  • Selected references: Dobakova et. al  (2007). Role of the PsbI Protein inPhotosystem II Assembly and Repair in the Cyanobacterium Synechocystis sp. PCC 6803 Plant Physiol 145:1681-1691.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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