Psb27-H1 | Photosystem II repair protein 27

Product no: AS22 4788

AS22 4788   | Clonality:  Polyclonal |  Host:  Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana PSB27-H1 protein sequence, UniProt: Q9LR64, TAIR: At1g03600
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum, in PBS pH 7.4
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution, add 50 µl, of sterile or deionized water.
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 18.8 | 11.7 | kDa (due to terminal processing)
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brassica oleracea, Brassica rapa,Capsella rubella, Coffea arabica,Camellia sinensis,Cucurbita pepo subsp. pepo, Erythranthe guttata,Gossypium hirsutum, Hevea brasiliensis, Hibiscus syriacu,Morus notabilis, Populus alba, Populus trichocarpa,Raphanus sativus, Quillaja saponaria
    Species of your interest not listed? Contact us
    Not reactive in: Chlamydomonas reinhardtii
  • Application Examples
  • Western blot using anti-Psb27-H1 antibodies
    ≈ 30 - 3.75 µg/well of total thylakoid protein of Arabidopsis thaliana, extracted freshly from previously isolated thylakoid membranes (2021/07/30, kept at -80°C).  Initial sample buffer: 20 mM MES-NaOH pH 6.3, 5mM MgCl2,15mM NaCl. Sample was denatured with Fast sample buffer: In short, to each 5μL aliquot of sample were added 3 µL Bio-Rad Native Page Sample buffer cat #161-0738 + 2 µL 10% SDS + 1 µL 2% β-mercaptoethanol) at 70°C for 5 minutes. Samples were separated on 4-20% SDS-PAGE and blotted for 45 minutes nitrocellulose membrane, using: semi-dry transfer. Blot was blocked with 5 % milk for: 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 in TBS-T with 5% milk ON/4°C with agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (Goat anti-rabbit IgG horse radish peroxidase conjugated, AS09 602 Agrisera) diluted to 1: 25 000 in TBS-T for 1h30/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent: AgriseraBright (AS16 ECL-N-10). Exposure time was 10 minutes.

    Courtesy of André Graça, Doctoral student at Department of Chemistry, Umeå University, Sweden

  • Additional Information
  • Additional information (application): Freshly extracted samples are recommended for the analysis. For protein transfer, use a membrane with a pore size of 0.2 µm to secure that the protein will transfer correctly, as described here.
  • Background
  • Background: Psb27-H1 (Photosystem II repair protein 27) is involved in repair of photodamaged photosystem II (PSII). Localized in the chloroplast lumen, and involved in cellular response to light intensity. Alternative name: Thylakoid lumenal protein PSB27-H1.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items