Psb29 | Thylakoid membrane formation 1, THF1

AS07 240 | Clonality: Polyclonal | Host: Rabbit | A.thaliana, H. vulgare, N. benthamiana

Psb29 | Thylakoid membrane formation 1, THF1

Product Information

Immunogen

KLH-conjugated peptide derived from known sequences of Psb29 including Arabidopsis thaliana Q9SKT0, At2g20890

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunoprecipitation (IP), Western blot (WB)

Recommended dilution 5 µg in 300 µl (IP), 1 : 10 000 (WB)

Expected | apparent MW 33,7 | 22 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana benthamiana

Predicted reactivity Nicotiana tabacum, Oryza sativa, Ostreococcus sp., Picea sitcHensis, Populus balsamifera, Physcomitrium patens, Solanum lycopersicum, Solanum tuberosum
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Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Background

Psb29 (THF1) is a conserved 22 kDa protein which functions in biogenesis of photosystem II complexes. This protein is required for organization of vesicles into mature thylakoid stacks for chloroplast development. Mediates G-protein signalling between the plasma membrane and the plastid.

Product citations

Selected references Hamel et al. (2016). The chloroplastic protein THF1 interacts with the coiled-coil domain of the disease resistance protein N' and regulates light-dependent cell death. Plant Physiol. 2016 Mar 7. pii: pp.00234.2016
Huang et al. (2013). Arabidopsis Thylakoid Formation 1 Is a Critical Regulator for Dynamics ofPSII-LHCII Complexes in Leaf Senescence and Excess Light. Mol Plant. May 13.