PsaA | PSI-A core protein of photosystem I

N-terminal part of recombinant PsaA protein from Chlamydomonas reinhardtii P12154

82 | 55-60 kDa

Chromera velia

Application example

2 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Hordeum vulgare leaf, (3) Chlamydomonas reinhardtii total cell, (4) Synechococcus sp. 7942 total cell all extracted with Protein Extration Buffer, PEB (AS08 300), were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2%  blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, frecommended secondary antibody AS09 602) diluted to 1:50 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescence detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 10 seconds.

PsaA is a hydrophobic protein and we recommend to use PVDF membrane for transfer to assure best results.

This product can be sold containing ProClin if requested.

Immunogold localization has been done in leaf material of  Arabidopsis thaliana.

Collection of antibodies to PSI proteins

recommended secondary antibody

Plant and algal protein extraction buffer

Secondary antibodies

PsaA is a core protein of photosystem I. In plants and cyanobacteria, the primary step in oxygenic photosynthesis, the light induced charge separation, is driven bytwo large membrane intrinsic protein complexes, the photosystems I and II.  Synonym: Photosystem I P700 chlorophyll a apoprotein A1.