PsbA | D1 protein of PSII, C-terminal (chicken)
AS01 016 | Clonality: Polyclonal | Host: Hen | Reactivity: [global antibody] for higher plants, algae, cyanobacteria and dinoflagellates
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|Recommended dilution||1 :4000-1 : 8000, 5 µg of total protein, (WB)|
|Expected | apparent MW||
38 | 28-30 kDa
|Confirmed reactivity||Alaria esculenta, Amphidinium carterae, Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlamydomonas raudensis (both Antarctic and mesophilic strains), Cyanophora sp. , Cynara cardunculus, Gonyaulax polyedra, Fucus vesiculosus, Horderum vulgare, Lobaria pulmonaria, Petunia sp. , Pinus sylvestris, Spartina alterniflora, Synechococcus sp. PCC 7942, Triticum aestivum, Ulva sp., symbiotic dinoflagellates of Stylophora pistillata and Turbinaria reniformis.|
Algae (brown and red), Conifers, Cryptomonads, Legumes, Stramenopiles, Euglenoids, Prochlorophytes, Xantophytes
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions.In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.
This antibody will also detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.
|Selected references||Dy et al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446.
Kim et al. (2018). The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves. Rice (N Y). 2018 Jan 5;11(1):1. doi: 10.1186/s12284-017-0196-8.
Yokono et al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.
Su et al. (2014). Exogenous progesterone alleviates heat and high light stress-induced inactivation of photosystem II in wheat by enhancing antioxidant defense and D1 protein stability. Plant Growth Regul DOI 0.1007/s10725-014-9920-1
Esparza et al. (2013). Katanin Localization Requires Triplet Microtubules in Chlamydomonas reinhardtii. PLOS one.
Hoogenboom et al. (2012). Effects of Light, Food Availability and Temperature Stress on the Function of Photosystem II and Photosystem I of Coral Symbionts. POLS one.
Morash et al. (2007). Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Canadian J. of Botany, 2007, 85(5): 476-483, 10.1139/B07-043.
2 µg of total protein from (1) Arabidopsis thaliana leaf extracted with PEB (AS08 300), (2) Horderum vulgare leaf extracted with PEB (AS08 300), (3) Chlamydomonas reinhardtii total cell extracted with PEB (AS08 300), (4) Synechococcus sp. 7942 total cell extracted with PEB (AS08 300), (5) Anabaena sp. total cell extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 50 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-hen IgY horse radish peroxidase conjugated, recommended secondary antibody AS09 603) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).
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