PsbA | D1 protein of PSII, DE-loop

AS10 704 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, C.reinhardii, H.vulgare, M.truncatula, N. oceanica, N. oleoabundans UTEX 1185 (chlorophyta), P.patens, P.sativum, S.vulgaris, S.alba, S.oleracea, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Triticum sp.

Product Information

Immunogen

KLH-conjugated synthetic peptide, amino acids 234-242 of Arabidopsis thaliana D1 protein UniProt: P83755, TAIR:AtCg00020

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Blue Native (BN-PAGE), Western blot (WB)

Recommended dilution 1 : 10 000, thylakoid fraction (WB)

Expected | apparent MW

38 | 28-30 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Chlamydomonas reinhardii, Hordeum vulgare, Medicago truncatula, Nannochloropsis oceanica strain IMET1, Neochloris oleoabundans UTEX 1185 (chlorophyta), Physcomitrella patens, Pisum sativum, Silene vulgaris, Sinapsis alba, Spinacia oleracea, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Triticum sp.

Predicted reactivity Cucumis sativus, Glycine max, Nannochloropsis sp., Nicotiana tabacum, Oryza sativa, Populus balsamifera, Ricinus communis, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application example

western blot using DE-loop antibody

Thylakoid membranes from Arabidopsis (0.05-0.75 µg of Chl) were separated on 14%AA+ 6M urea gels and blotted 1h to PVDF. Blots were blocked immediately following transfer in 5% milk solution for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 20 000 o.n. at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody, HRP conjugated, diluted to 1:5 000. The blots were washed as above and developed for 5 min with ECL-Plus detection reagent according to the manufacturers instructions. Exposure time was 1 min in CCD camera Fuji4000.

Courtesy Professor Cornelia Spetea-Wiklund, University of Ghotenburg, Sweden

Additional information

Additional information Antibody will detect 23 kDa N-terminal fragment

Antibody is recognizing a 23 kDa fragment in spinach and Arabidopsis thylakoidsfor usage on total cell extracts the dilution needs to be determined experimentally.

Related products

AS05 084 | Anti-PsbA antibody, C-terminal, rabbit antibodies

AS01 016 | Anti-PsbA C-terminal, chicken antibodies

AS06 124A | Anti-PsbA, N-terminal, rabbit antibodies

AS01 016S | PsbA protein standard for quantitation and positive control

Plant and algal protein extraction buffer

Secondary antibodies

Background

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1