PsbA | D1 protein of PSII, DE-loop
AS10 704 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, C.reinhardii, H.vulgare, M.truncatula, N. oceanica, N. oleoabundans UTEX 1185 (chlorophyta), P.patens, P.sativum, S.vulgaris, S.alba, S.oleracea, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Triticum sp.
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38 | 28-30 kDa
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Thylakoid membranes from Arabidopsis (0.05-0.75 µg of Chl) were separated on 14%AA+ 6M urea gels and blotted 1h to PVDF. Blots were blocked immediately following transfer in 5% milk solution for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 20 000 o.n. at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody, HRP conjugated, diluted to 1:5 000. The blots were washed as above and developed for 5 min with ECL-Plus detection reagent according to the manufacturers instructions. Exposure time was 1 min in CCD camera Fuji4000.
Courtesy Professor Cornelia Spetea-Wiklund, University of Ghotenburg, Sweden
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1
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