Name:
Phone:
E-mail:
Address:






PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide)

AS05 092  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, H. vulgare, N. tabacum, P. sativum, Z. mays

PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide) in the group Antibodies Plant/Algal  / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS05 092)
PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide)



DATA SHEET IN PDF

Qty: 
381
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Customer reviews

Product Information

Immunogen

N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 and PsbO2 isoforms. UniProt: P23321 , PsbO1, TAIR: At5g66570 ; UniProt:A0A178VBH5, TAIR: At3g50820

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

33 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Manihot esculenta, Nicotiana tabacum, Pisum sativum, Sinapsis alba, Triticum aestivum, Zea mays
Predicted reactivity Brassica oleracea, Pisum sativum, Populus tremula, Picea sitcHensis, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in

Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

Application examples

Application examples

Application example

western blot detection using anti-PsbO antibody

2 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Horderum vulgare leaf ), (3) Chlamydomonas reinhardtii total cell , (4) Synechococcus sp. 7942 total cell were all extracted with PEB (AS08 300) and separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% blocking reagent in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in 2% blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with chemiluminescence detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). 

Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 24663344

Journal: J Exp Bot

Figure Number: 5G

Published Date: 2014-06-01

First Author: Casanova-Sáez, R., Mateo-Bonmatí, E., et al.

Impact Factor: 6.088

Open Publication

Subcellular and suborganellar localization of ANU10. (A–F) Confocal micrographs of the subepidermal layer of palisade mesophyll cells from (A–C) Ler and (D–F) anu10-1 35Spro:ANU10:GFP transgenic plants. Micrographs show (A, D) the chlorophyll autofluorescence of the chloroplasts, (B, E) the GFP fluorescence, and (C, F) an overlay of the chlorophyll and GFP signals, showing their co-localization in (F). Pictures were taken from first-node leaves collected 16 das. Scale bars indicate 50 ?m. (G) Western blot analysis of the proteins in chloroplast, stroma, and thylakoid fractions isolated from anu10-1 35Spro:ANU10:GFP transgenic plants collected 16 das. Primary antibodies against GFP, the large Rubisco subunit (RbcL), and the PsbO subunit of PSII were used. Molecular mass markers are indicated on the right.


Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 25835989

Journal: PLoS One

Figure Number: 7A

Published Date: 2015-04-04

First Author: Fristedt, R., Martins, N. F., et al.

Impact Factor: 2.942

Open Publication

Altered protein accumulation and stability of the chloroplast ATP synthase in the cgl160 mutant visualized by immunoblotting.A. Immunoblots with antibodies against essential subunits of the photosynthetic protein complexes of wild-type (Col-4) Arabidopsis and the two cgl160 T-DNA insertion lines grown under long-day and short-day conditions. Isolated thylakoid membranes were used, and equal amounts of chlorophyll were loaded onto the SDS-PAGE gel. For approximate quantification, wild-type samples from long-day plants were diluted to 10%, 25% and 50%, respectively. Accumulation of PSII was probed with antibodies against PsbB and PSBO. Additionally, the PSBS protein involved in NPQ and the minor PSII antenna protein LHCB4 were probed. Accumulation of the cytochrome b6f complex was probed with antibodies against the essential subunits PetA (cytochrome f), PetB (cytochrome b6), and PETC (Rieske protein). Accumulation of PSI was probed with antibodies against the reaction center subunit PsaB and the stromal ridge subunit PsaD. ATP synthase accumulation was probed with antibodies against the CF1 subunits AtpA (CF1?), AtpB (CF1?) and AtpD (CF1?) and antibodies against the CF0 subunits AtpF (CF0b) and AtpI (CF0a). B. Loading difference estimation for immunoblotting CF1 between wild type and cgl160-1. To obtain similar immunoblotting signal three times more (15 ?g protein) was needed for cgl160-1 compared to wild type (5 ?g protein). C. Maintenance of CF1 was measured by incubating leaves from wild type and cgl160-1 in solution containing the plastid protein synthesis inhibitor chloramphenicol for the indicated time points. Protein extract was isolated and separated by SDS-PAGE, immunoblotted and probed with specific antibodies against CF1 and LHCB2.1. Three times more protein was loaded from the mutant to obtain equal level of CF1 immunoblotting signal, as specified in B.


Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 26442058

Journal: Front Plant Sci

Figure Number: 6B

Published Date: 2015-10-07

First Author: Murgia, I., Giacometti, S., et al.

Impact Factor: 5.435

Open Publication

O2 evolution and protein profile of photosynthetic apparatus in A. thaliana seedlings with generational exposure to Fe deficiency. (A) SHR-trap 1445 c0, pH 7.7 s1 pH 7.7 s2, pH 7.7 s1 c2 seedlings were grown for 11 days in control AIS medium and net O2 evolution (expressed as ?mol O2 evolved min-1 mg chlorophyll-1) was measured under illumination at either 100 or 800 ?E m-2 s-1. Values are mean ą SE of three biological replicas, each consisting of at least 15 seedlings. Letters represent statistical differences, according to Student’s t-test, with p < 0.05. (B) Western blot analysis with antibodies against PsaA, CP43, PsbO, D1, LHCII, and PsbE of thylakoid membranes purified from 11 days-old SHR-trap 1445 c0 seedlings germinated in -Fe AIS medium or from 11 days-old SHR-trap 1445 c0, pH 7.7 s1, pH 7.7 s1 pH 7.7 s2, pH 7.7 s1 c2 seedlings germinated in control (+Fe) AIS medium. Samples corresponding to 1 ?g chlorophyll were loaded on each lane.


Reactant: Plant

Application: Western Blotting

Pudmed ID: 27590049

Journal: BMC Plant Biol

Figure Number: 9A

Published Date: 2016-09-02

First Author: Mazur, R., Sadowska, M., et al.

Impact Factor: 4.142

Open Publication

Changes of PSII and PSI antenna and core protein levels. Proteins from control and Tl-treated white mustard leaves were separated by SDS-PAGE followed by immunodetection with antibodies against Lhcb1, Lhcb2, Lhca1 (antenna proteins) and D1, D2, CP43, PsbO, PsaA (core proteins). Samples were loaded on the equal amount of chlorophyll (0.25 ?g). Description of samples abbreviation as given in the legend to Fig. 3


Reactant: Arabidopsis thaliana (Thale cress)

Application: Western Blotting

Pudmed ID: 28791032

Journal: Front Plant Sci

Figure Number: 2A

Published Date: 2017-08-10

First Author: Kohzuma, K., Froehlich, J. E., et al.

Impact Factor: 5.435

Open Publication

Changes in the protein levels of photosynthetic components under extended dark exposure in wild-type and gamera-1. Immunoblot detection of photosynthetic proteins from leaves of Ws and gamera-1 plants incubated after dark adaptation for 0, 2, and 4 days was examined. Specifically, essentially thylakoid fractions were assayed to determine the content of the following proteins: ?-subunit of ATP synthase; the D1 protein, OEC17, OEC23, and OEC33 of PSII; Cyt f and Rieske protein of the cytochrome b6f complex; and the F and D subunits of PSI, after extended dark treatment. Proteins were resolved via SDS-PAGE gel based on equal microgram chlorophyll per lane loading and processed as described in Section “Materials and Methods”. The Large subunit of RuBisco and LHCII stained with either CBB or Ponceau red, respectively, are presented here as loading controls. DAD indicates days after dark adaptation.

Additional information

Additional information

Loading based on 50-100 ng of chlorophyll is enough to obtain good signal with this antibody

Good signal is obtained with this antibody with a load from 0,5 chlorophyll ĩg/well

Related products

Related products AS14 2824 | Anti-PsbO1 | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS14 2825 | Anti-PsbO2 | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS06 142-33 | Anti-PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS06 167 | Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS08 305 | Anti-PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

AS06 142-16 | Anti-PsbQ | 16 kDa protein of the oxygen evolving complex (OEC) of PSII, rabbit antibodies

Plant protein extraction buffer 

Background

Background

The PsbO protein is an extrinisic subunit of the water splitting photosystem II (PSII) complex. The protein is exposed on the luminal side of the thylakoid membrane, and is hihgly conserved in all known oxygenic photosynthetic organisms. Alternative names of PsbO1 include  33 kDa subunit of oxygen evolving system of photosystem II, OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 1 and for PsbO2 33 kDa subunit of oxygen evolving system of photosystem II,  OEC 33 kDa subunit, 33 kDa thylakoid membrane protein, manganese-stabilizing protein 2.

Product citations

Selected references Mazur et al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187(4):2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.
Toubiana et al. (2020). Correlation-based Network Analysis Combined With Machine Learning Techniques Highlight the Role of the GABA Shunt in Brachypodium Sylvaticum Freezing Tolerance. Sci Rep , 10 (1), 4489
Wang et al. (2019). YR36/WKS1-mediated Phosphorylation of PsbO, an Extrinsic Member of Photosystem II, Inhibits Photosynthesis and Confers Stripe Rust Resistance in Wheat. Mol Plant. 2019 Oct 14. pii: S1674-2052(19)30330-2. doi: 10.1016/j.molp.2019.10.005.
An et al. (2019). Protein cross-interactions for efficient photosynthesis in the cassava cultivar SC205 relative to its wild species. J Agric Food Chem. 2019 Jul 19. doi: 10.1021/acs.jafc.9b00046.
Rozp?dek et al. (2018). Acclimation of the photosynthetic apparatus and alterations in sugar metabolism in response to inoculation with endophytic fungi. Plant Cell Environ. 2018 Dec 5. doi: 10.1111/pce.13485.
Li et al. (2018). Comparative proteomic analysis of key proteins during abscisic acid-hydrogen peroxide-induced adventitious rooting in cucumber (Cucumis sativus L.) under drought stress. Journal of Plant Physiology Volume 229, October 2018, Pages 185-194.
Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016 Sep 2;16(1):191. doi: 10.1186/s12870-016-0883-4.
Albanese et al.(2016). Isolation of novel PSII-LHCII megacomplexes from pea plants characterized by a combination of proteomics and electron microscopy. Photosynth Res. 2016 Jan 9.
Hu et al. (2015). Site-specific Nitrosoproteomic Identification of Endogenously S-Nitrosylated Proteins in Arabidopsis. Plant Physiol. 2015 Feb 19. pii: pp.00026.2015.
Fristedt et al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.
Casanova-Sáez et al. (2014). Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development. J Exp Bot. 2014 Mar 24.
Albus et al. (2012). LCAA, a novel factor required for Mg protoporphyrin monomethylester cyclase accumulation and feedback-control of aminolevulinic acid biosynthesis in tobacco. Plant Physiol. Oct 19.

Related products: PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS16 ECL-N |  low pico to mid femtogram detection

This product can be purchased in 3 ...
From 12 €
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
199 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €