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PsbN | Potosystem II reaction center protein N

AS14 2786 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PsbN | Potosystem II reaction center protein N in the group Antibodies for Plant/Algal  / Photosynthesis  / PSII (Photosystem II) at Agrisera AB (Antibodies for research) (AS14 2786)

DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Customer reviews

Product Information

Immunogen

KLH-conjugated synthetic peptide chosen from PsbN protein of Arabidopsis thaliana Uniprot: P62113, TAIR: AtCg00700

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

4.7 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabacum
Predicted reactivity Arabis alpina, Camelia sp., Canna indica, Cannabis sativa, Costus pulverulentus, Glycine max, Helianthus tuberosus, Hordeum vulgare, Lactuca sativa, Lilium sp., Manihot esculenta, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Populus trichocarpa, Saccharum officinarum, Solanum tuberosum, Sorghum timorense, Spinacia oleracea, Tricitum aestivum, Thaumatococcus daniellii, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-PsbN antibodies

5 µg of membrane proteins from Nicotiana tabacum WT and Δ psbN mutant isolated with homogenization buffer (50 mM Tris HCl pH 8.0, 10 mM EDTA, 2 mM EGTA, 10 mM DTT ) were separated on 15% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blots were blocked with 3% BSA for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1000 for 12h at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, AS09 602, Agrisera) diluted to 1:25 000 in BSA for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 53 seconds.

Courtesy of Dr. Jörg Meurer, Biozentrum der Ludwig-Maximilians-Universität München, Germany

Additional information

Additional information This product can be sold containing 0.1% ProClin

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Background

Background

The PsbN protein is chloroplast-encoded, low molecular weight protein annotated as a photosystem II subunit however it seems that it is not a constituent subunit of PSII but is required for PSII repair from photoinhibition. 

Product citations

Selected references Torabi et al. (2014). PsbN Is Required for Assembly of the Photosystem II Reaction Center in Nicotiana tabacum. Plant Cell. 2014 Mar;26(3):1183-99. doi: 10.1105/tpc.113.120444. Epub 2014 Mar 11.

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