Agrisera blog

Agrisera Best Poster Prize awarded during Epichrome 2020 in Ume

Agrisera at Epichrome 2020

Agrisera participated in the Epichrome 2020 meeting taking place at Umeå University, Sweden, between 27.02-28.02. 

Our warmest congratulations to Dr. Eva Brinkman from Pelechano Lab at SciLifeLab, who was awarded the Agrisera Best Poster Prize at this meeting. The poster title was: "Genetic wiring maps of co-acting transcriptional factors in single cells".

Awaiting your free antibody choice!

Agrisera collection for epigenetic research can be found here.

Read more 2020-02-29

Agrisera's educational poster 4 RUBISCO

Rubisco Agrisera Educational Poster 4

Download the poster here

Poster 4:
Rubisco: An enzyme of Global Importance, provides a good overview on the most abundant enzyme on Earth - Rubisco. 

The poster is developed by Dr Dmitry Shevela (Umeå University, Sweden),  Dr. Manajit Hayer-Hartl (Max Planck Institute of Biochemistry, Germany) , Professor Inger Andersson (Uppsala University, Sweden) and Professor Govindjee (University of Illinois at Urbana-Champaign, USA), and consulated with leading scientists in the field. This poster is available in A0 format, for decoration your office or lab wall, as well as in pdf format for lectures and workshops. E-Mail us to obtain your hard copy!

Agrisera Educational Poster Collection includes following posters:

Oxygenic Photosynthesis (Poster 1)
Z-scheme (Poster 2)
Photosynthesis and Respiration (Poster 3)

All posters can be aquired at several conferences, which Agrisera is attending or supporting this year.
For a list of these conferences, please click here.

Agrisera Photosynthetic Antibody Collection consists of antibodies for:

Rubisco Quantification | ATPsynthase | Carotenoid metabolism | Chaperones/Transporters | Chloroplast biogenesis/architecture | Chlorophyll | Green Cut | LHC | Light acclimation | Kinases | PSI (Photosystem I) | PSII (Photosystem II) | Proteases | Protein standards-quantitation | RUBISCO/carbon metabolism | Sucrose metabolism
Read more 2020-02-27

Use Agrisera kits to quantify PSI, PSII, Rubisco

Agrisera offers several combinations of antibodies to: Rubisco, PSI and PSII proteins, which together with calibrated protein standards can be used to quantify major photosynthetic proteins.

Photosynthesis Tool Kit – quantitation, PSI, PSII, Rubisco, 

(Agrisera), AS04 051

Western Blot Rubisco quantitation kit

(Agrisera), AS09 409

Beside primary antibodies and quantification standards, the kits also contain matching secondary antibodies of high titer, min. 1> 25 000 1h/RT (AS09 602) and suitable chemiluminescent detection reagent, AgriseraBright in mid picogram range. 


Example


Figure 1: Immunoblot of samples of Synechococcus elongatus PCC 7942 (lanes 1-4) and Anabaena sp. PCC 7120 (lanes 5-8). Molecular weight markers (MagicMark XP, Invitrogen) are in lane 9. Recombinant PsbA protein standards are loaded in lanes 10-12 at 0.05 pmoles, 0.15 pmoles and 0.45 pmoles.

The blot was quantitated with QuantityOne software and the standard curve is shown in Figure 2.


standard curve

Figure 2: Standard curve for the blot in Figure 1. Picomoles of quantitated standard is plotted on the X-axis versus the adjusted signal volume obtained on the Y-axis.

Quantitative Western Blot method is described here. You are also welcome to watch a short video tutorial

Read more 2020-02-25

Agrisera Best Poster Prize awarded during Solar Energy to Biomass conference in Portugal

Agrisera Best Poster Prize on SEB22020 meeting in Portugal

Our warmest congratulations to PhD candidate Xin Liu from Vrije University of Amsterdam, The Netherlands, who was awarded the Agrisera Best Poster Prize during the Solar Energy to Biomass conference in Porto, Portugal, 11.02-14.02. The title of the poster is: "Chlamydomonas reinhardtii Exhibits De Facto Constitutive NPQ Capacity in Physiologically Relevant Conditions".

The Agrisera antibody collection to Chlamydomonas reinhardii proteins can be found here.
Read more 2020-02-19

Next poster is coming in Agrisera educational poster collection

Agrisera Educational Poster 4 - Rubisco


This month, the Poster 4 of the Agrisera Educational Poster series is going to be launched. The title of this poster is Rubisco: "An Enzyme of Global Importance".
The poster is currently being consulted with several specialists in the field of photosynthesis.
Previous posters from the Agrisera poster collection can be viewied here

All posters can be requested as hard copies by sending a message to: [email protected].
Read more 2020-02-17

Most published antibody to most abundant protein on Earth - Rubisco

Rubisco - most abundant protein on Earth

Rubisco is an amazing protein, and also the most abundant protein on Earth. Agrisera has provided specific antibodies to this enzyme for over 17 years. These antibodies have been cited in thousands of publications and used on a very wide range of species. The Agrisera anti-RbcL form I antibodies are made to a very conserved, 7 amino acid long peptide, and is therefore categorized as a Global Antibody. The specific peptide used is present in Rubisco form I from higher plants, algae and cyanobactera:

Anti-RbcL I | Rubisco large subunit, form I antibodies (AS03 037) - provided at a 50 % discount in February.

Combined with the Rubisco protein standard (AS01 017S) and the Rubisco Western Blot quantitation kit (AS09 409), this antibody can be used for quantification of Rubisco using quantitative western blot method. A short video tutorial for this method can be found here

For photosynthetic bacteria, archea and dinoflagellates, Agrisera offers another antibody: 

Anti-RbcL II | Rubisco large subunit, form II antibodies (AS15 2955) - provided at a 50 % discount in February.

This antibody can be combined with RbcL II quantification standard (AS15 2955S) and applied to quantify RbcL II. 

Agrisera's Rubisco antibody collection also consists of other useful antibodies, like: RbcS (AS07 259A), Rubisco activase (AS10 700), Rubisco chaperones and other proteins. The whole collection can be found here

Good luck with your research!
Read more 2020-02-10
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