Agrisera blog

Oligomer specific amyloid-beta antibodies
Discover Agrisera's unique monoclonal antibodies targeting amyloid-beta oligomers. The approach is based on a combination of high avidity and cryptic epitopes and result in a high selectivity for Abeta oligomers where oligomers are selectively detected also in the presence of stoichiometric excess of abeta monomers corresponding to more than 4 orders of magnitude.

The approach is based on a sandwich ELISA set up where the oligomer-specific antibodies are immobilized in the bottom, followed by exposure to the sample of interest. The oligomer specific antibodies will bind the oligomers with a very high binding strength, and detection of bound abeta oligomers is subsequently performed by a normal pan anti-abeta antibody. For the second step, we recommend strong binding polyclonal anti-abeta antibody or biotin labelled variants of the oligomer-specific monoclonals.

Suggested antibody pairs with excellent performance are indicated below:

Suggested sandwich ELISA pair for the detection abeta-oligomers:

ELISA setup 1
Coating OMAB, mouse monoclonal antibody (AS10 932)
Detection OMAB, Biotinylated (AS10 932B), or amyloid-beta peptide rabbit antibody (AS08 328)

ELISA setup 2
Coating: Anti-mAB-M | Mouse anti-human Abeta protein (3-10) region, oligomer-specific (clone 2D10,F6) mouse monoclonal antibodies (AS13 2716) or Anti-mAB-O | Mouse anti-human Abeta protein (3-10) region, oligomer-specific (clone 3E5,F8) mouse monoclonal antibodies (AS13 2715)

Detection: OMAB, Biotinylated (AS10 932B), or amyloid-beta peptide rabbit antibody AS08 328)

The method used to develop these antibodies is described in detail in:  Brännström et al. 2014, PLoS one: A Generic Method for Design of Oligomer-Specic Antibodies.

Other antibodies (not oligomer specific) include: 

Anti-Abeta (1-42) | Amyloid-beta peptide 1-42, rabbit antibodies (AS08 328)
Anti-Abeta (1-42) | Amyloid-beta peptide 1-42, rabbit antibodies (AS08 357)
Anti-Abeta (1-42) | Amyloid-beta peptide 1-42, chicken antibodies (AS08 329)

Other antibodies to support Alzheimer's research include: 

Anti-Alpha-1-antichymotrypsin, goat antibodies (AS10 1317)

Anti-APP Delta C31 (C-terminal), rabbit antibodies (AS21 4571)
Anti-APP | Amyloid Precursor Protein (N-term), rabbit antibodies (AS20 4455)
Anti-APP | Amyloid Precursor Protein (C-term), rabbit antibodies (AS20 4456)
Anti-APP-C31 | Caspase 3 cleaved APP, C terminal fragment, rabbit antibodies (AS21 4570)

Anti-BDNF | Brain-derived neurotrophic factor, rabbit antibodies (AS13 2691)

Anti-ChAT | choline O-acetyltransferase, rabbit antibodies (AS13 2689)

Anti-GLO1 | Glyoxalase I (clone 6F10), rat antibodies (AS21 4568)

Anti-IAPP | Human IAPP (amylin) 1-37, specific for the native hormone having a disulphide-bridge between Cys2-Cys7, chicken antibodies (AS08 359)
antibodies specific for peptides with an intact disulfide bridge

Anti-PEN2 | gamma-secretase subunit PEN-2, rabbit antibodies (AS06 154)

Anti-Prealbumin | TTR transthyretin, goat polyclonal antibodies (AS10 1326)

Anti-Transthyretin 39-44, amyloid specific , mouse monolclonal antibodies (AS16 3112)
Anti-Transthyretin 56-61, amyloid specific (mouse monoclonal antibody), mouse monoclonal antibodies (AS16 3113)

Anti-VRL-1, rabbit polyclonal antibodies (AS13 2690)

The Global Plant Events Calendar was initiated on January 2nd, 2019 as a mutual effort of Plantae and Agrisera, to provide the plant community with a comprehensive list of worldwide events. Over the last three years, hundreds of various events have been listed in the calendar, making it easy for plant researchers to receive a quick overview of available events. As the pandemic developed in 2020, many events were cancelled, postponed, or moved online. All these changes were regularly updated in the calendar, which became a source for information on in-person, as well as online meetings, like webinars on different topics relevant to plant biology. 

Today, The Global Plant Events Calendar is the most comprehensive list of worldwide events for the plant community.

If you are not familiar with the calendar, this short video will show you how to use it. You are welcome to submit information about a meeting you are organizing!

Stay informed! Follow the calendar on Twitter @PlantSciEvents

Save your protocol processing time and use directly conjugated primary antibodies!

Agrisera offers chosen primary antibodies conjugated to:

• Alkaline phosphatase (ALP) 
• Biotin
• Horse radish peroxidase (HRP)

Using a directly conjugated primary antibody shortens the protocol time, by omitting washing and secondary antibody incubation steps. 

Choose amoing the following antibodies:

Anti-aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker) - ALP conjugated, AS09 580-ALP
Anti-aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker) - Biotin conjugated, AS09 580B
Anti-aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker) - HRP conjugated, AS09 580-HRP

Anti-AGO1 | Argonaute 1 - ALP-conjugated, AS09 527-ALP
Anti-AGO1 | Argonaute 1 - Biotin conjugated, AS09 527B
Anti-AGO1 | Argonaute 1 - HRP-conjugated, AS09 527-HRP

Anti-ASyO2-biotinylated | Mouse anti-human alpha-synuclein | oligomer-specific, AS13 2717B

Anti-BC2-tag - ALP conjugated AS20 4446-ALP
Anti-BC2-tag - Biotin conjugated AS20 4446B
Anti-BC2-tag - HRP conjugated  AS20 4446-HRP

Anti-BiP | Lumenal-binding protein, HRP-conjugated, AS09 481-HRP
Anti-BiP | Lumenal-binding protein, ALP-conjugated, AS09 481-ALP
BiP | Lumenal-binding protein, Biotin-conjugated, AS09 481B

Anti-BirA (mutated/TurboID)  - ALP conjugated, AS20 4440-ALP
Anti-BirA (mutated/TurboID)  - Biotin conjugated, AS20 4440B
Anti-BirA (mutated/TurboID)  - HRP conjugated, AS20 4440-HRP

Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal) - ALP conjugated, AS16 3690-ALP
Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal) - Biotin conjugated, AS16 3690B
Anti-Cas9 | CRISPR-associated endonuclease 9 (polyclonal) - HRP conjugated, AS16 3690-HRP

Anti-Clathrin heavy-chain 1,2,  HRP-conjugated, AS10 690-HRP
Anti-Clathrin heavy-chain 1,2, ALP-conjugated, AS10 690-ALP
Anti-Clathrin heavy-chain 1,2, Biotin-conjugated, AS10 690B

Anti-C-YFP | C-terminal of YFP - ALP conjugated, AS11 1775-ALP
Anti-C-YFP | C-terminal of YFP - Biotin conjugated, AS11 1775B
Anti-C-YFP | C-terminal of YFP - HRP conjugated, AS11 1775-HRP

Anti-GFP  |  Green fluorescent protein (VENUS) - ALP conjugated, AS18 4227-ALP
Anti-GFP  |  Green fluorescent protein (VENUS) - Biotin conjugated, AS18 4227B
Anti-GFP  |  Green fluorescent protein (VENUS) - HRP conjugated, AS18 4227-HRP

Anti-GFP | Green Fluorescence Protein - ALP conjugated, AS20 4443-ALP
Anti-GFP | Green Fluorescence Protein - Biotin conjugated, AS20 4443B
Anti-GFP | Green Fluorescence Protein - HRP conjugated, AS20 4443-HRP

Anti-GST-tag (rabbit antibody, polyclonal) - ALP conjugated, AS17 4147-ALP
Ant-GST-tag (rabbit antibody, polyclonal) -  Biotin conjugated, AS17 4147B
Anti-GST-tag (rabbit antibody, polyclonal) - HRP conjugated, AS17 4147-HRP

Anti-H3 | Histone H3, ALP-conjugated, AS10 710-ALP
Anti-H3 | Histone H3, HRP-conjugated, AS10 710-HRP
Anti/H3 | Histone H3, Biotin-conjugated, AS10 710B

Anti-His-tag | 3xHis (polyclonal) - ALP conjugated, AS20 4441-ALP
Anti-His-tag | 3xHis (polyclonal) - Biotin conjugated, AS20 4441B
Anti-His-tag | 3xHis (polyclonal) - HRP conjugated, AS20 4441-HRP

Anti-KLH | Keyhole limpet hemocyanin, ALP-conjugated, AS99 001-ALP
Anti-KLH | Keyhole limpet hemocyanin, HRP-conjugated, AS99 001-HRP 
Anti-KLH | Keyhole limpet hemocyanin, Biotin-conjugated, AS99 001B 

Anti-LUC | Luciferase (firefly)  - ALP conjugated, AS16 3691-ALP
Anti-LUC | Luciferase (firefly) - Biotin conjugated, AS16 3691B
Anti-LUC | Luciferase (firefly) - HRP conjugated, AS16 3691-HRP

Anti-PEPC | phosphoenolpyruvate carboxylase, ALP-conjugated, AS09 458-ALP
Anti-PEPC | phosphoenolpyruvate carboxylase, HRP-conjugated, AS09 458-HRP
Anti-PEPC | phosphoenolpyruvate carboxylase, Biotin-conjugated, AS09 458B

Anti-PIP (PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5) aquaporins, ALP-conjugated, AS09 487-ALP
Anti-PIP (PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5) aquaporins, HRP-conjugated, AS09 487-HRP
Anti-PIP (PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5) aquaporins, Biotin-conjugated, AS09 487B

Anti-PRK ribulose-5-P-kinase | phosphoribulokinase, ALP-conjugated, AS07 257-ALP
Anti-PRK ribulose-5-P-kinase | Phosphoribulokinase, HRP-conjugated, AS07 257-HRP
Anti-PRK ribulose-5-P-kinase | phosphoribulokinase, Biotin-conjugated, AS07 257B

Anti-PsbA | D1 protein of PSII, C-terminal, ALP-conjugated, AS05 084A-ALP
Anti-PsbA | D1 protein of PSII, C-terminal, HRP-conjugated, AS05 084A-HRP
Anti-PsbA | D1 protein of PSII, C-terminal, Biotin-conjugated, AS05 084B

Anti-RbcL | Rubisco large subunit, form I, ALP-conjugated, AS03 037-ALP
Anti-RbcL | Rubisco large subunit, form I, Biotin conjugated, AS03 037B
Anti-RbcL | Rubisco large subunit, form I, HRP-conjugated, AS03 037-HRP

Anti-SPS | Sucrose phosphate synthase, global, ALP-conjugated, AS03 035-ALP
Anti-SPS | Sucrose phosphate synthase, global, HRP-conjugated, AS03 035-HRP
Anti-SPS | Sucrose phosphate synthase, global, Biotin-conjugated, AS03 035B

Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5, ALP-conjugated, AS07 212-ALP
Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5, HRP-conjugated, AS07 212-HRP
Anti-VDAC1-5 | Voltage-dependent anion-selective channel protein 1-5, Biotin-conjugated, AS07 212B

Use discount code: Conj20
Valid January-June 2022