Abeta (1-42) | Amyloid-beta peptide 1-42

AS08 328 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human

Product Information

Immunogen synthetic peptide chosen from human Abeta (1-42) protein, Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V-I-A

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Dot Blot (Dot), ELISA (ELISA), Immunolocalization (IL), Western blot (WB)

Recommended dilution 1 : 1000 (DB), 1 : 3000 (ELISA), 1-2 µg/ml (IL)

Expected | apparent MW 4,5 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity Bovine, Chicken, Dog, Porcine, Rabbit

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Dot blot experiment

• Membrane: Nitrocellulose
• Blocking buffer: 5% dry milk in PBS, 0.1% Tween 20
• Antibody dilution: 1:1000
• Secondary antibody: anti-rabbit (HRP)
• Detection: Enhanced Chemoluminescence (pico) 30s

*Abeta (1-42) consists of both monomeric and partly aggregated mtrl.

Additional information

The antibody can detect Abeta (1-42), Abeta (1-28) Abeta (1-20) and Abeta (1-17), This product exhibits a low reactivity to monomeric Abeta (1-42) as determined by SDS-PAGE and Western blotting,Immunolocalization: human tissue was paraffin-embedded and sectioned, De-waxed and rehydrated in an ethanol gradient, Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h, The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit

Background

Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein P05067

Product citations

Selected references Lindhagen-Persson et al. (2010). Amyloid-β oligomer specificity mediated by the IgM isotype--implications for a specific protective mechanism exerted by endogenous auto-antibodies. PLoS One. 2010 Nov 10;5(11):e13928. doi: 10.1371/journal.pone.0013928.

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