APP Delta C31 (C-terminal)

Human NT2 neurons (neurally differentiated human NT2 embryonic carcinoma cells) were infected with adenovirus vector expressing beta-galactosidase (1), wild-type APP (2) or APPdelta C31 (3). Cell lysates were prepared 48 h after infection, and proteins were analysed by Western blotting. Neurons overexpressing wild-type APP contained a 96 kD SAC-immunoreactive fragment which was also detected in APP delta C31-overexpressing neurons.

Neurally differentiated NT2 cells were cultured for 96 h in the absence of fetal calf serum. Cells were triply labeled for MAP2, the neuronal marker microtubule-associated protein 2 (MAP2), chromosomal DNA (Hoechst), and APP delta C31 (SAC). MAP2-immunopositive neurons with apoptotic nuclei (arrows) are intensively labelled with anti-APP delta C31 antibodies. The cells were fixed with 4 % formaldehyde and blocked with 4 % non-fat milk and 5 % goat normal serum at 4°C/ON.