APP-C31 | Caspase 3 cleaved APP, C terminal fragment
AS21 4570 | Clonality: | Host: Rabbit | Reactivity: Human, mouse, rat
|Data sheet||Product citations||Protocols||Add review|
Sample: NT2 neurons
Fixation: 72 h after infection with adenovirus vector expressing Amyloid Precursor Protein Anti-APP-C31 antibody was used in dilution 1: 1000.
Left image: stained for the N-terminus of APP with P2-1, mouse monoclonal antibody specific for N-terminus of APP.
Center image: stained for chromosomal DNA (Hoechst). Right image: stained for C-terminus of the caspase 3 cleaved APP with anti-APP-C31 antibody (ACT1).
Most of APP-accumulating neurons with shrunken and fragmented nuclei contain ACT1-immunoreactivity (arrows), but non-neuronal cells are hardly labeled with ACT1 (arrowheads).
The tissue was fixed with 4 % formaldehyde and permalized with acetone at -20ºC, followed by blocking with 4 % non-fat milk and 5 % goat normal serum at 4°C/ON. Primart antibody was incubated at 1: 1000 1h/RT.
Related products: APP-C31 | Caspase 3 cleaved APP, C terminal fragment
This product can b...