APP-C31 | Caspase 3 cleaved APP, C terminal fragment

AS21 4570 | Clonality: | Host: Rabbit | Reactivity: Human, mouse, rat

Product Information

Immunogen Synthetic peptide corresponding to the N-terminal of human caspase 3-generated APP C-terminal 31 amino acids (aa 665-670) UniProt: P05067

Host

Rabbit

Purity Serum. Contains 0.05 % sodium azide.

Format Liquid

Quantity 100 µl

Reconstitution For reconstitution add µl, of sterile water

Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunolocalisation (IL), Western blot (WB)

Recommended dilution 1::500 - 1: 1000 (IL), 1:1000-1:3000 (WB)

Expected | apparent MW 86,9 | 100 kDa

Reactivity

Confirmed reactivity Human, Mouse, Rat

Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Immunolocalization using anti-APP-C31 polyclonal antibodies

Sample: NT2 neurons
Fixation: 72 h after infection with adenovirus vector expressing Amyloid Precursor Protein Anti-APP-C31 antibody was used in dilution 1: 1000.
Left image: stained for the N-terminus of APP with P2-1, mouse monoclonal antibody specific for N-terminus of APP.
Center image: stained for chromosomal DNA (Hoechst). Right image: stained for C-terminus of the caspase 3 cleaved APP with anti-APP-C31 antibody (ACT1).
Most of APP-accumulating neurons with shrunken and fragmented nuclei contain ACT1-immunoreactivity (arrows), but non-neuronal cells are hardly labeled with ACT1 (arrowheads).

The tissue was fixed with 4 % formaldehyde and permalized with acetone at -20ºC, followed by blocking with 4 % non-fat milk and 5 % goat normal serum at 4°C/ON. Primart antibody was incubated at 1: 1000 1h/RT.

Additional information

Background

Background The Alzheimer Amyloid Precursor Protein (APP) is a transmembrane protein whose abnormal processing is associated with the pathogenesis of Alzheimer’s disease. APP695 lacking the protease inhibitor domain is the predominant form in neuronal tissues. APP695 is cleaved by caspases into the 664-residue amino (N)-terminal fragment that lacks the carboxyl C-terminal 31-residues (APPC31) and the 31-residues C-terminal fragment (APP-C31). Both fragments might be potent inducers of neuronal apoptosis. An antibody (named ACT1) against the N-terminus of caspase 3-generated APP C-terminal 31 aa of human APP695 (APP-C31 ) was raised in rabbit.

Product citations

Selected references Nishimura et al. (2002) Cell death induced by a caspase-cleaved transmembrane fragment of the Alzheimer amyloid precursor protein. Cell Death Differ. 2002 Feb;9(2):199-208. doi: 10.1038/sj.cdd.4400931. PMID: 11840170.

background:	The Alzheimer Amyloid Precursor Protein (APP) is a transmembrane protein whose abnormal processing is associated with the pathogenesis of Alzheimer’s disease. APP695 lacking the protease inhibitor domain is the predominant form in neuronal tissues. APP695 is cleaved by caspases into the 664-residue amino (N)-terminal fragment that lacks the carboxyl C-terminal 31-residues (APPC31) and the 31-residues C-terminal fragment (APP-C31). Both fragments might be potent inducers of neuronal apoptosis. An antibody (named ACT1) against the N-terminus of caspase 3-generated APP C-terminal 31 aa of human APP695 (APP-C31 ) was raised in rabbit.
Confirmed reactivity:	Human, Mouse, Rat
predicted reactivity:	Species of your interest not listed? Contact us
calculated \| apparent molecular mass [kDa]:	86,9 \| 100 kDa
Format:	Liquid
Host:	Rabbit
immunogen:	Synthetic peptide corresponding to the N-terminal of human caspase 3-generated APP C-terminal 31 amino acids (aa 665-670) UniProt: P05067
Purity:	Serum. Contains 0.05 % sodium azide.
Quantity:	100 µl
recommended dilution:	1::500 - 1: 1000 (IL), 1:1000-1:3000 (WB)
Reconstitution:	For reconstitution add µl, of sterile water
storage:	Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunolocalisation (IL), Western blot (WB)
Picture (footer):	Sample: NT2 neurons Fixation: 72 h after infection with adenovirus vector expressing Amyloid Precursor Protein Anti-APP-C31 antibody was used in dilution 1: 1000. Left image: stained for the N-terminus of APP with P2-1, mouse monoclonal antibody specific for N-terminus of APP. Center image: stained for chromosomal DNA (Hoechst). Right image: stained for C-terminus of the caspase 3 cleaved APP with anti-APP-C31 antibody (ACT1). Most of APP-accumulating neurons with shrunken and fragmented nuclei contain ACT1-immunoreactivity (arrows), but non-neuronal cells are hardly labeled with ACT1 (arrowheads). The tissue was fixed with 4 % formaldehyde and permalized with acetone at -20ºC, followed by blocking with 4 % non-fat milk and 5 % goat normal serum at 4°C/ON. Primart antibody was incubated at 1: 1000 1h/RT.
All references:	Nishimura et al. (2002) Cell death induced by a caspase-cleaved transmembrane fragment of the Alzheimer amyloid precursor protein. Cell Death Differ. 2002 Feb;9(2):199-208. doi: 10.1038/sj.cdd.4400931. PMID: 11840170.

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