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Abeta (1-42) | Amyloid-beta peptide 1-42

AS08 357  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Human

Abeta (1-42) | Amyloid-beta peptide 1-42 in the group Antibodies Human Cell Biology / Neuroscience / Neurodegenerative diseases / Alzheimer's disease at Agrisera AB (Antibodies for research) (AS08 357)



DATA SHEET IN PDF

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333
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Product Information

Immunogen synthetic peptide chosen from human Abeta (1-42) protein, Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V-I-A
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Dot Blot (Dot), ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 1000 (Dot), 1 : 3000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW 4,5 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Bovine, Chicken, Dog, Porcine, Rabbit
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Dot blot and Western analysis
• Membrane: Nitrocellulose
• Blocking buffer: 5% dry milk in PBS, 0.15% Tween 20
• Antibody dilution : 1:1000
• Secondary antibody: anti-rabbit (HRP)
• Detection: Enhanced Chemoluminescence (pico) 10s


Additional information

The antibody can detect Abeta(1-42), Abeta(1-28) Abeta(1-20) and Abeta(1-17)

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Background

Background

Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of  the amyloid precursor protein P05067

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