Abeta (1-42) | Amyloid-beta peptide 1-42
AS08 357 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human
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Product Information
Immunogen
synthetic peptide chosen from human Abeta (1-42) protein. Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V-I-A
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
100 µl
Reconstitution
For reconstitution add 100 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Dot Blot (Dot), ELISA (ELISA), Western blot (WB)
Recommended dilution
1 : 1000 (Dot), 1 : 3000 (ELISA), 1 : 1000 (WB)
Expected | apparent MW
4.5 kDa
Reactivity
Confirmed reactivity
Human
Predicted reactivity
Bovine, Chicken, Dog, Porcine, Rabbit
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
Dot blot and Western analysis
• Membrane: Nitrocellulose
• Blocking buffer: 5% dry milk in PBS, 0.15% Tween 20
• Antibody dilution : 1:1000
• Secondary antibody: anti-rabbit (HRP)
• Detection: Enhanced Chemoluminescence (pico) 10s
• Membrane: Nitrocellulose
• Blocking buffer: 5% dry milk in PBS, 0.15% Tween 20
• Antibody dilution : 1:1000
• Secondary antibody: anti-rabbit (HRP)
• Detection: Enhanced Chemoluminescence (pico) 10s
Additional information
The antibody can detect Abeta(1-42), Abeta(1-28) Abeta(1-20) and Abeta(1-17).
Background
Background
Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein P05067
Product citations
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