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RbcL | Rubisco large subunit, form I, Biotin conjugated (40 ”g)
AS03 037B | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, licHens, algae, cyanobacteria, dinoflagellates, diatoms compartment marker of chloroplast stroma in higher plants and cytoplasm in cyanobacteria
Benefits of using this antibody
- Product Info
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Immunogen: KLH-conjugated synthetic peptide conserved across all known plant, algal and (cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), including Arabidopsis thaliana AtCg00490, Hordeum vulgare P05698, Oryza sativa P0C510, Chlamydomonas reinhardtii P00877, Synechococcus PCC 7920 A5CKC5
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Liquid Quantity: 40 ”g Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Tested applications: Western blot (WB) Recommended dilution: 1 : 2000 - 5 000 (WB) Expected | apparent MW: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Apium graveolens, Artemisia annua, Baculogypsina sphaerulata (benthic foraminifer), Bienertia sinuspersici, Cicer arietinum, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Cyanophora paradoxa, Cylindrospermopsis raciborskii CS-505, Emiliana huxleyi, Euglena gracilis, Fraxinus mandshurica, Fucus vesiculosus, Glycine max, Gonyaulax polyedra, Guzmania hybrid, Heterosigma akashiwo, Karenia brevis (C.C.Davis) s) G.Hansen & Ű.Moestrup (Wilson isolate), Liquidambar formosana, Micromonas pusila, Nicotiana benthamiana, Physcomitrium patens, Porphyra sp. , Schima superba, Stanleya pinnata, Spinacia oleracea, lichens, Symbiodinium sp., Synechococcus PCC 7942, Thalassiosira pseudonana, Thermosynechococcus elongatus, Prochlorococcus sp. (surface and deep water ecotype), Triticum aestivum, dinoflagellate endosymbionts (genus Symbiodinium), extreme acidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum strain SolV, Thalassiosira punctigera, Vitis vinifera Predicted reactivity: Aalpha proteobacteria, Algae (brown and red), Dicots, Beta-proteobacteria, Conifers, Cryptomonads, Cyanobacteria (prochlorophytes), Gamma-proeobacteria, Liverworts, Monocots, Mosses, Suaeda glauca, Welwitschia
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: Anti-RbcL can be used as a cellular [compartment marker] of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31,25 fmoles, As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants, This antibody together with Agrisera Rubisco protein standard is very suitable to quantify Rubisco in plant and algal samples - Background
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Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Accessories
AS01 017S | Protein standard for quantitation and positive control
AS03 037A | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, lichens, algae, cyanobacteria, dinoflagellates, diatoms compartment marker of chloroplast stroma in higher plants and cytoplasm in cyanobacteria
Benefits of using this antibody
AS07 259 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, C. pepo, C. reinhardtii, C. sativus, H. vulgare, M. domestica, N. tabacum, S. lycopersicum
