Streptavidin HRP Conjugated
- Data sheet
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Streptavidin is a protein that has a very high affinity for biotin (known as vitamin B7 or H). The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is mostly used in molecular biology and bionanotechnology since the complex of streptavidin-biotin is resistant to organic solvents, denaturants etc. and extremes of temperature and pH.
- Product Info
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Quantity: 1 mg Storage: Store freeze-dried (lyophilized) powder at 2-8°C. Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily prior to use and then discard.Shelf life of this product is 1 year from date of receipt. Tested applications: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) Recommended dilution: 1 : 500-1 : 2000 (IHC), 1 : 5 000- 1: 50 000 (ELISA), (WB) - Additional Information
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Additional information (application): Buffer composition: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % Kathon CG (v/v) is included as preservative.
Streptavidin was overexpressed in bacterial culture, purified and conjugated to horse radish peroxidase (HRP). - Background
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Background: Streptavidin HRP conjugate is designed to be used in the detection of biotinylated primary antibodies.
This product is ELISA grade. - Protocols
- Antibody protocols
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Accessories
- 10ml trial size is restricted to one unit per customer -
- 10ml trial size is restricted to one unit per customer -
This product is designed to stabilize coated ELISA plates and other solid phase substrates.
AS18 MacroStab | Agrisera Universal Stabilizer preserves and stabilizes the activity of HRP or ALP conjugates and proteins and other bio-molecules. It is designed as a ready to use stabilizer, buffer, diluent and possible coat block for immunoassays.
This product can be purchased in 3 different volumes:
AS18 Macrostab-10, 10 ml. Trial size limited to one per customer
AS18 Macrostab-100, 100 ml
AS18 Macrostab-1L, 1L
Choose the appropriate volume in the drop down menu to the right
AS15 Diluent | TMB based, especially formulated with extreme sensitivity, HRP substRate for microwell application.