Streptavidin HRP Conjugated, for ELISA
AS15 2860 | conjugate is suitable for the detection of biotinylated primary antibodies

Data sheet | Product citations | Protocols | Add review |
Streptavidin is a protein that has a very high affinity for biotin (known as vitamin B7 or H). The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Streptavidin is mostly used in molecular biology and bionanotechnology since the complex of streptavidin-biotin is resistant to organic solvents, denaturants etc. and extremes of temperature and pH.
Product Information
Quantity
1 mg
Storage
Store freeze-dried (lyophilized) powder at Store at 2-8°C. Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.Shelf life of this product is 1 year from date of receipt.
Tested applications
This conjugate is designed to be used in the detection of biotinylated primary antibodies. It is suitable for use in ELISA (ELISA), WB, IHC.
Recommended dilution
The optimal working dilution should be determined by the investigator.Suggested starting dilution(s):1 : 500-1 : 2000 1 : 50 000-1 : 5 000 (WB)
Reactivity
Application examples
Additional information
Buffer composition: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % Kathon CG (v/v) is included as preservative.
Streptavidin is overexpressed in bacterial culture, purified and conjugated to horse radish peroxidase (HRP).
Background
Product citations
Related products: Streptavidin HRP Conjugated, for ELISA
AS18 AgriCoat-Block | Non Mammalian Protein Coat Block (5x)
- 10ml trial size is restricted to on...
- 10ml trial size is restricted to on...
From 13 €
AS18 MacroStab | Agrisera Universal Stabilizer preserves and stabilizes the activity o...
From 13 €
AS15 Diluent | TMB based, especially formulated with extreme sensitivity, HRP sub...
80 €
AS15 TMB-HRP | TMB based, especially formulated with extreme sensitivity, HRP substrate for microw...
From 10 €