AgriseraTMB Diluent (100 ml)
AS15 Diluent | TMB based, especially formulated with extreme sensitivity, HRP substRate for microwell application.
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After incubations with antibodies or HRP labeled reagents, TMB solution is added. As an alternative TMB can also be spiked with a different volume of buffered HRP to create dilution series of this substrate, for example 50 %, 70%. This will change sensitivity of this product and can make it more suitable for certain assays, depending what kinetics are required. If you require to adjust sensitivity of your assay, please check Agrisera TMB/Diluent pack.
The oxidation of TMB by HRP produces a blue reaction product, measured at 650 nm. Color formation can be recorded as a function of time or the reaction can be stopped using an equal volume of 0.3 M sulfuric acid after a fixed interval. Increased sensitivity is achieved by converting the blue radical to the yellow diimine by addition of acid. The resulting yellow chromogen is measured immediately at 450 nm.