AgriseraTMB HRP Substrate
- 10ml trial size is restricted to one unit per customer -
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After incubations with antibodies or HRP labeled reagents, TMB solution is added. As an alternative TMB can also be spiked with a different volume of buffered HRP to create dilution series of this substrate, for example 50 %, 70%. This will change sensitivity of this product and can make it more suitable for certain assays, depending what kinetics are required. If you require to adjust sensitivity of your assay, please check Agrisera TMB/Diluent pack.
The oxidation of TMB by HRP produces a blue reaction product, measured at 650 nm. Color formation can be recorded as a function of time or the reaction can be stopped using an equal volume of 0.3 M sulfuric acid after a fixed interval. Increased sensitivity is achieved by converting the blue radical to the yellow diimine by addition of acid. The resulting yellow chromogen is measured immediately at 450 nm.
How to work with this reagent?
- Pour out amount of reagent which is needed for your assay.
- Do not our back any unused substrate back to the original bottle.
- Any trace metals, HRP or other contaminant may be reason for the substrate to turn blue.