AgriseraTMB HRP Substrate (1L)
- 10ml trial size is restricted to one unit per customer -
- Product Info
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Quantity: 10 ml 100 ml 1L Storage: Stable for 3 years at +2-8° C or at room temperature up to six months, and can also be frozen. Storge at room temperature for 1 month results in ca, 2 % activity loss. Protect the substrate from exposure to sunlight. Tested applications: Instructions for a 96 well micro titer plate 1, Equilibrate the substrate to the assay temperature before use, 2, Complete all required incubations with antibodies and HRP labeled reagents, 3, Wash the plate at least 4 times with phosphate buffered saline or Tirs buffered saline containing 0,1% Tween-20, THen shake and blot all residual buffer from plate wells, 4, Add 100 ”l of the TMB solution to each well and incubate 5-30 minutes, depending upon the activity of the HRP probe, If color develops too rapidly, zero order kinetics will not prevail, Dilution of antibody, or HRP-labeled reagent may be required, As alternative Agrisera TMB/Diluent pack can be used, 5, To stop the reaction use:-equial volume of 0,3 M sulfuric acid (100 ”l), The yellow chromogen in the stopped reaction should be read at 450 nm, This will provide highest sensitivity of detection, -Monitoring the assay kinetics at may be done at 650 nm as a function of time, The reaction can be stopped to preserve the blue chromogen using an equal volume, 100 ”l of 0,1% sodium fluoride or 0,15% sodium dodecyl sulfate, The blue chromogen in the stopped reaction should be measured at 650 nm, Adjustments of time, reagent volume and temperature require standardization by the end user, In case of any further questions please contact us, - Additional Information
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Additional information: One component ready to use reagent. It contains 2.5 mMol L-1 TMB and Hydrogen Peroxide in a buffer at pH 3.6. It also contains non-toxic stabilizers.
How to work with this reagent?
- Pour out amount of reagent which is needed for your assay.
- Do not our back any unused substrate back to the original bottle.
- Any trace metals, HRP or other contaminant may be reason for the substrate to turn blue.
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Accessories
AS15 Diluent | TMB based, especially formulated with extreme sensitivity, HRP substRate for microwell application.
AS15 HRPstab | Bovine and mammalian protein free HRP and ALP-conjugate and protein stabilizer
This product can be purchased in 2 different volumes:
AS15 HRPstab-10, 10 ml. Trial size limited to one per customer
AS15 HRPstab-100, 100 ml
Choose the appropriate volume in the drop down menu to the right
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