Agrisera blog

Thank you to all our new and old customers for being with us during 2010, sharing your ideas, giving suggestions and helping us to grow up Agrisera catalogue.

Agrisera protein extraction buffer PEB and global antibodies (anti-PsbA and anti-RbcL) were used to validate high throughput protein extraction from tough, recalcitrant marine phytoplankton.

Standard microprobe sonication/flash freeze protocol has been compared with Precellys® (Bertin Technologies) bead-based system to extract total proteins from multiple samples in parallel.

Comparison of ribulose-1,5-bisphosphate carboxylase oxygenase (RbcL) immunodetection from phytoplankton, after protein extraction through sonication or Precellys bead beating. RbcL was detected by immunoblotting using a chicken anti-RbcL primary antibody (AS01 017) and a horseradish peroxidase-conjugated goat anti-chicken secondary antibody (AS09 603). MW, molecular weight protein ladder (in kDa).

The total yield of proteins extracted from each species was similar using the sonication/flash freeze protocol or Precellys® bead beating system. Using Precellys® system, sample throughput and reproducibility are higher than with the sonication/flash freeze protocol.