Agrisera blog

Multispecies reactivity of Agrisera antibodies

Many of Agrisera antibodies have a wide range of reactivity - in total covering few hounderds of different species including di, monocots, ferns, mosses, algae and diatoms. Majority of our antibodies are developed to 7-15 amino acid sequences which are carefully chosen to allow multi-species detection (see our Antibody Production Guide). Such wide reactivity is however not always possible, depending upon which protein part is targeted.

Therefore, our product information sheets are including information about confirmed (based on experimental data) and predicted reactivity (based on sequence conservation). In cases when you are not certain or your species is not listed on our product information sheet - please contact us providing a protein sequence you aim to detect in your assay and information in what assay you are planning to use our antibody. Some antibodies will not work in techniques which require binding to a native and exposed part of a protein.

Agrisera antibodies multispecies detectopm

Following those links, you can find out which of Agrisera antibodies are reacting with chosen model species: Arabidopsis thaliana | Chlamydomonas reinhardii | cyanobacteria | Conifers | diatoms | Glycine max | Hordeum vulgare | Medicago truncatula | Nicotiana tabacum | Oryza sativa | Phaseolus vulgaris | Physcomitrella patens | Pisum sativum | Populus sp. | Solanum lycopersicum | Solanum tuberosum | Spinacia oleracea | Triticum sp. | Zea mays | Vitis vinifera | Search an antibody by species reactivity here.
Read more 2014-02-17

Agrisera's reference record is growing

Last week's PSL Alliance published "Research Antibody Market Report" in association with Antibodypedia. As you can see in the table prepared in the report, Agrisera's growth based on publication record places us between large companies on the world antibody market. We continue to work hard to provide the community with high quality, well validated primary and secondary antibodies.

Agriseras growth based on reference record

The PSL Alliance is a new network for SMEs supplying reagents for research use only. The aim of the Alliance is to help promote manufacturers of high quality products.
Read more 2014-02-10

How to know that a commercial antibody is going to work on a protein from my species?

Agrisera Antibodies Commercial antibodies are developed to various antigens (more information about procedures can be found in Agrisera Antibody Production Guide), often being recombinant proteins of full length, truncated or 7-20 amino acid long peptides. Development of antibodies against specific peptides is a common approach for cases where a specific isoform of a protein family is to be targeted. Therefore, such an antibody will often be rather isoform and species-specific.
Based on antigen sequence, it is possible to predict if the antibody in question has the potential to work on a protein from species not listed on the product information sheet. Agrisera product information sheets list both confirmed reactivity (based on experimental results), and predicted reactivity (resulting from sequence alignment of the peptide sequence used to elicit given antibody). 

If information regarding a specific sequence used to elicit given antibody is not available - please contact us with your question, and provide us with the protein sequence you are aiming to detect. Even if there is some conservation level found in protein alignment, it is crucial to know if the sequence of the antigen used to elicit a given antibody is conserved in your protein sequence, and to what extent.

This will save you money and time in the future. You are welcome to contact us with your question!
Read more 2014-02-06
 Agrisera blog


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