Agrisera blog

I have purchased several antibodies from the same company, and they all worked on my species. Therefore the next one should work too!


Each antibody is made to a different protein and thus recognizes different epitopes. Therefore, even if many antibodies purchased from a given company worked well for your species, this does not guarantee that the next antibody will. There needs to be a match of the sequence the antibody is binding to in the sequence of the protein which is aimed to be detected. Based on our experience, at least a 70 % match in linear sequence needs to be confirmed. Many antibodies are made to short, 10 to 15 amino acids long peptides. Overall target protein conservation between species will not be relevant in such a case if there is no match in the exact sequence used to elicit the given antibody. 



Therefore, before purchasing an antibody which has not been used on a given species or application, conservation of the antigen used to elicit such antibody should be confirmed.

Send your question to: [email protected] and we will help to check sequence conservation and confirm your choice for the best outcome of your experiments. 

Our warmest congratulations to Dr. Manuela Leonardelli from Roman Ulm lab at University of Geneva, who received the Agrisera Best Poster Prize for her poster: "UVR8 - mediated photoprotection in Arabidopsis thaliana" during the Plants in Changing World meeting in Helsinki, Finland.

Awaiting your antibody choice!

Agrisera Photosynthetic antibody collection covers various aspects of photosynthesis and offers a broad species reactivity.

Our warmest congratulations to PhD candidate Artem Iakunkov, from the Department of Physics, Umeå University, Sweden, who received The Best Poster Prize funded by Agrisera at the KBC Days 2019. The poster title was: "Strong effect of ageing on swelling properties of graphene oxide membranes in alcohols".
The awardee has also received a jar of honey made by KBC bees.

KBC Days is a yearly event at the Chemistry and Biology House at Umeå University. The programme this year included inspiring presentations by new faculty members and award recipients, and 11 poster presentations from midterm PhD students. Guided tours to present the infrastructures/platforms were also offered.

Thank you for this fruitful and interesting event!

Can listings a product numbers of primary and secondary antibody used in your research actually minimize waste of work and resources in the future?

More than half of the antibodies currently used in research cannot be properly identified, which makes it impossible to conduct a given experiment using the same set of antibodies. Companies often offer multiple antibodies to the same target protein (e.g. tags and protein modifications) and identifying a specific antibody used in a given publication is simply impossible. 

There are no worldwide standard guidelines on how antibodies should be cited in publications and journals. Besides the product number for any primary antibody used in research, the secondary antibody should also be properly cited with product number. As primary and secondary antibodies in a successful experiment create a unique pair, such result may not be reproducible with another secondary antibody. Antibodies differ in their characteristics, and lots of time and money can be saved in the future if product numbers of both primary and secondary antibodies are listed in all publications. 

You can also read about this topic in Citeab blog post: "Don’t forget about citing your secondaries!"