Agrisera blog

Agrisera Best Poster Prizes at ASPB Northeastern Section 2023 Meeting

Agrisera supported the ASPB Northeastern Section 2023 Meeting, held between the 22nd and 23rd of April, 2023, in SUNY Old Westbury, USA. The meeting was organized ny Prof. Christos Noutsos.

The winners of Agrisera Best Poster Prizes, and a free antibody each from the Agrisera catalog, were:

Cassandra Probert from Samuel Hazen’s Lab at the University of Massachusetts, with the poster "BdSND2R is a transcriptional repressor that forms a negative feedback loop with SWAM1 to control secondary wall thickening in Brachypodium distachyon"


Alaa Mohamed Elsaeed Hassan Elminisy from Om Prakash Dhankher lab at  the University Massachusetts Amherst, with the poster "Molecular and Biochemical studies on the interaction between cucumber and Plant Growth Promoting Rhizobacteria (PGPR) under Cadmium (Cd) Stress".

We are awaiting your free antibody choice!
 Awardees of Agrisera Best Poster Prize at ASPB Northeastern Section 2023 Meeting
Left to right: Cassandra Probert, Professor Christos Noutsos and Alaa M. El-Minis.

Read more 2023-04-27

Comprehensive collection of antibodies to Arabidopsis thaliana proteins involved in photosynthesis

Antibodies to Arabidopsis thaliana proteins involved in photosynthesis are a core collection of the Agrisera catalog. This group of products has been developed in collaboration with research laboratories worldwide, for the last 20 years. Agrisera photosynthetic antibodies have the following features:

  • Developed to well-conserved peptides, which allows for broad species reactivity
  • Can be used at high dilutions, which allows many tests to be conducted
  • Validated in independent laboratories, with appropriate sets of endogenous samples (no recombinant protein validation only)
  • Available since the year 2000, meaning this collection is prided by an extensive publication record
  • For your convenience, some of the antibodies to major photosynthetic proteins, are put together into sets, at discounted pricing. In some cases, these are also accompanied by quantitation standards, to allow estimation of PSI/PSII ratio.

Beside antibodies, Agrisera has also developed Educational Posters on Photosynthesis, that are engaging and packed with up-to-date information. These posters are available for download, and as hard copies that can be requested here. Each poster was created in consultation with leading researchers from the scientific community, and the information in the posters is updated regularly. Specific figures and schemes from the posters are available free of charge for download, to be used in publications and presentations.

 Thylakoid membrane

Read more 2023-04-27

Agrisera Best Poster Prize at the conference of Austrian Society of Plant Biology (ATSPB)

Agrisera supported the 24th conference of the Austrian Society of Plant Biology (ATSPB), held between the 13th and 15th of April, 2023, in Hall in Tirol, Austria. The event was organized by the Department of Botany of the University of Innsbruck.

The 24th conference of the ATSPB was dedicated to the importance of plants in the context of food security, biodiversity and climate change. The Agrisera Best Poster Prize was awarded to Paraskevi Charalambous, from the University of Natural Resources and Life Sciences, Vienna (BOKU), Austria, for the poster "Dissolving and Reshaping Walnut Shells: A Sustainable Material Solution?".

Awaiting your free antibody choice!
   Agrisera prize at ATSPB conference 2023
In the picture: Paraskevi Charalambous (left) and Prof Ilse Kranner, Conference President (right).

Read more 2023-04-19

New species reactivity added for anti-PsbO antibodies

Anti-PsbO antibodies, product number: AS06 142-33, detect a PsbO protein in a wide range of different species.

Recently, reactivity on a new species, Eucalyptus grandis, has been experimentally confirmed using the following protocol:

13.5 µg/well of total protein extracted freshly from Eucalyptus sp. leaves.  Exact buffer components were:  50 mM HEPES NaOH pH 8.0, 5mM DTT, 15 mM NaHCO3, 20 mM MgCl2, 2 mM EDTA, 4% (v/v) EDTA- free protease inhibitor cocktail (Roche Diagnostics), 4% PVPP (w/v), 20% glycerol (v/v), 1:1 Tris-buffered phenol (v/v) and 100 mM CH3COONa in methanol. Extracts were denatured with SDS beta-Mercaptoethanol sample buffer for 10 min at 90°C. Samples were separated at RT on 12% SDS-PAGE and blotted for 1 h to PVDF, using wet transfer at room temperature (RT). Blot was blocked with 5% non-fat milk for 1 h at RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:5000 for 1 h at RT with agitation in TBS-T. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed three times for 10 min with TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG HRP, Agrisera AS09 602) diluted to 1: 10000 in TBS-T for 1 h at RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent. Exposure time was 12 min.
   Anti-PsbO antibodies with reactivity to Eucalyptus

1: 13.5 µg of Eucalyptus grandis whole leaf extract clone one (g1)
4: 13.5 µg of Eucalyptus grandis whole leaf extract clone two (g2)
6: 13.5 µg of one clone Eucalyptus grandis x Eucalyptus camaldulensis whole leaf extract (gc)
8: 13.5 µg of one clone Eucalyptus grandis x Eucalyptus tereticornis whole leaf extract (gt)

Presented data courtesy of Ing. Agr. MSc. Matías Nión from Departamento de Biología Vegetal, Facultad de Agronomía, UdelaR. Montevideo, Uruguay.

Read more 2023-04-17

How to choose the correct antibody?

With millions of antibodies offered worldwide, by hundreds of suppliers, it may be a bit overwhelming, especially for beginners, to choose the correct antibody to answer a specific questions in a research project. Here is a short checklist with what to pay attention to:

Species reactivity – Has the antibody been used to detect the protein from the species we are working with? If not, a request should be sent to the supplier to confirm if the antibody may have so-called predicted reactivity to the target protein. If not, the antibody is not suitable, and false positive results can be produced.  

Validation data – Is validation data available on a product information sheet? For non-essential proteins, validation data should include results obtained with knockdown or knockout mutants. Western blot results showing that an antibody binds to a recombinant protein, are not relevant to the binding in a specific sample, extracted from a specific tissue or organism. Here we explain why.

Application – If the antibody has only been used in Western blot, it may not work in immunoprecipitation or immunolocalization. Each of these techniques requires separate validation data.

Recommended antibody dilution – The lower the dilution, the fewer tests can be conducted using an antibody. Here we explain why

Publication record – Being citet in scientific articles definitely confirms the performance of a given antibody. However, as there is no worldwide standard, product numbers are oftentimes not included in the "Material and Method" section. This makes it difficult to identify what antibody has been used to obtain the presented results. How to cite antibodies in scientific publication, is explained in this short video.

  Primary antibody

You are always welcome to contact us with any questions, and we will promplty help you to find the best antibody for your specific species and application. 

Read more 2023-04-14

Agrsera Best Poster Awards at the 19th International Workshop on Plant Membrane Biology

The 19th International Workshop on Plant Membrane Biology (IWPMB) took place Taipei, Taiwan, between 27th and 31st of March. This meeting has been organized since 1968 and covers topics related to membrane transport in the context of signaling, physiology, biotic and abiotic stresses and development. 

Agrisera supported the meeting with two Best Poster Prizes.

The Best Poster Prizes were awarded for the scientific poster contribution of Huimin Feng from Nanjing Agricultural University, the poster title was "P02-11 Strigolactone and gibberellin signalling coordinately regulates metabolic adaptations to changes in nitrogen availability",

and Dr. Swee-Suak Ko from Academia Sinica, Biotechnology Center in Southern Taiwan with the poster "P11- 11 Redefine the Role of OsPHO1;2 Phosphate Transporter in Rice Grain Filling".
We are awaring your free antibody choices! Good luck with your research! 

Agrisera Best Poster Award at 19th International Workshop on Plant Membrane Biology

Read more 2023-04-14

Marker antibodies for Arabidopsis thaliana cellular compartments

Well-validated antibodies to specific cellular compartments are crucial to support data regarding localization studies of novel proteins. Agrisera's Cellular compartment antibody collection contains of marker antibodies to:

Cell wall
| Chloroplast | Cytoplasm | Microsomal membranes | Mitochondria | Nuclei | Peroxisomes | Plasma membrane | Vacuole

Agrisera compartment marker antibodies have been used in in a wide range of publications over the last 15 years. For many compartments we offer several antibodies, to proteins of different molecular weight, to match your experimental requirements.

Not certain which compartment marker antibody to choose? Ask Agrisera's techincal staff for an advice.  

Until end of June 2023, Agrisera Antibody Collection for Arabidopsis thaliana is provided with a 10% discount


Read more 2023-04-06
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