Agrisera blog

Antibodies to Arabidopsis thaliana proteins involved in photosynthesis are a core collection of the Agrisera catalog. This group of products has been developed in collaboration with research laboratories worldwide, for the last 20 years. Agrisera photosynthetic antibodies have the following features:

• Developed to well-conserved peptides, which allows for broad species reactivity
• Can be used at high dilutions, which allows many tests to be conducted
• Validated in independent laboratories, with appropriate sets of endogenous samples (no recombinant protein validation only)
• Available since the year 2000, meaning this collection is prided by an extensive publication record
• For your convenience, some of the antibodies to major photosynthetic proteins, are put together into sets, at discounted pricing. In some cases, these are also accompanied by quantitation standards, to allow estimation of PSI/PSII ratio.

Beside antibodies, Agrisera has also developed Educational Posters on Photosynthesis, that are engaging and packed with up-to-date information. These posters are available for download, and as hard copies that can be requested here. Each poster was created in consultation with leading researchers from the scientific community, and the information in the posters is updated regularly. Specific figures and schemes from the posters are available free of charge for download, to be used in publications and presentations.

Samples: 

1: 13.5 µg of Eucalyptus grandis whole leaf extract clone one (g1)
4: 13.5 µg of Eucalyptus grandis whole leaf extract clone two (g2)
6: 13.5 µg of one clone Eucalyptus grandis x Eucalyptus camaldulensis whole leaf extract (gc)
8: 13.5 µg of one clone Eucalyptus grandis x Eucalyptus tereticornis whole leaf extract (gt)

Presented data courtesy of Ing. Agr. MSc. Matías Nión from Departamento de Biología Vegetal, Facultad de Agronomía, UdelaR. Montevideo, Uruguay.

With millions of antibodies offered worldwide, by hundreds of suppliers, it may be a bit overwhelming, especially for beginners, to choose the correct antibody to answer a specific questions in a research project. Here is a short checklist with what to pay attention to:

Species reactivity – Has the antibody been used to detect the protein from the species we are working with? If not, a request should be sent to the supplier to confirm if the antibody may have so-called predicted reactivity to the target protein. If not, the antibody is not suitable, and false positive results can be produced.  

Validation data – Is validation data available on a product information sheet? For non-essential proteins, validation data should include results obtained with knockdown or knockout mutants. Western blot results showing that an antibody binds to a recombinant protein, are not relevant to the binding in a specific sample, extracted from a specific tissue or organism. Here we explain why.

Application – If the antibody has only been used in Western blot, it may not work in immunoprecipitation or immunolocalization. Each of these techniques requires separate validation data.

Recommended antibody dilution – The lower the dilution, the fewer tests can be conducted using an antibody. Here we explain why. 

Publication record – Being citet in scientific articles definitely confirms the performance of a given antibody. However, as there is no worldwide standard, product numbers are oftentimes not included in the "Material and Method" section. This makes it difficult to identify what antibody has been used to obtain the presented results. How to cite antibodies in scientific publication, is explained in this short video.

Well-validated antibodies to specific cellular compartments are crucial to support data regarding localization studies of novel proteins. Agrisera's Cellular compartment antibody collection contains of marker antibodies to:

Cell wall | Chloroplast | Cytoplasm | Microsomal membranes | Mitochondria | Nuclei | Peroxisomes | Plasma membrane | Vacuole

Agrisera compartment marker antibodies have been used in in a wide range of publications over the last 15 years. For many compartments we offer several antibodies, to proteins of different molecular weight, to match your experimental requirements.

Not certain which compartment marker antibody to choose? Ask Agrisera's techincal staff for an advice.  


Until end of June 2023, Agrisera Antibody Collection for Arabidopsis thaliana is provided with a 10% discount.