The dilution of a primary antibody will differ depending on several parameters, such as:
The product information sheet, provided with each antibody, contains suggestions regarding initial dilution in which a given antibody can be used. Agrisera antibodies are optimized for Western blot, and the recommended starting dilution is from 1: 1000, up to 1: 100 000, depending on the primary antibody. | ![]() Questions? Contact us Note: Each technique requires separate optimization, and due to target protein abundance and distribution, more concentrated primary antibodies need to be used in immunolocalization experiments. |
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Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?2023-09-01 I have not used this antibody before, what conditions of Western blot do you recommend?
2023-07-27 Which subclasses of immunoglobulin G are found in rabbit serum?
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