Antibodies can be made to a variety of different so-called antigens, like short peptides (5 to 20 amino acids), proteins, hormones and substances. Agrisera's product information sheets list confirmed and predicted reactivity. Confirmed reactivity means that there are experimental data supporting that this antibody detects the target protein from the listed species. Predicted reactivity means that there is potential for a given antibody to work, based on an antigen sequence homology check. Sequence homology is assessed in the linear protein sequence of subsequent amino acids, and needs to be at least 70 % for an antibody to work. If an antibody is made to a short peptide, overall sequence homology is not relevant. Please keep in mind that in cases where an antibody with predicted reactivity is used, a sample from a species with confirmed reactivity should be included in initial experiments. In some cases, the extraction buffer used for Western blot needs to be modified, depending on which species sample is extracted. Find an antibody for your experiments using Antibody Search by Species | ![]() Not certain if an antibody may work for your species of interest? Contact us |
Latest
Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?2023-09-01 I have not used this antibody before, what conditions of Western blot do you recommend?
2023-07-27 Which subclasses of immunoglobulin G are found in rabbit serum?
2023-07-18 Antibody suddenly does not work any longer, what can be the reason?
2023-07-11 Can an antibody made to a full-length protein distinguish between 2 isoforms?
2023-05-10 What is a reason for so called "ghost bands" to appear on blots?
2023-03-30 One of the ways to remove background on a Western blot
2023-03-24 How to predict in which technique a given antibody may work?
2023-03-10 Which capture antibody to use in ELISA?
2023-03-06 Why should we measure protein concentration?
2023-03-03
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