Agrisera blog

Agrisera at Nordic Photosynthesis Congress and Algal Symposium in Ume

Agrisera participated in the Nordic Photosynthesis Congress and Nordic Algae Symposium, between the 19th and 21st of June, at Umeå University, Sweden. At the event there were 119 researchers present, and 32 posters covering this year's theme "From Photosynthesis to Industrial Applications".

A group of conference participants from Germany and Finland visited Agrisera's facility in Umeå during the evening of the second day of the event. 

Two Agrisera Best Poster Prizes, and a free antibody each from the Agrisera catalog, were awarded to:
  • Lenny Maliham-Yap from the Institute of Molecular Biotechnology, Graz University of Technology in Austria for the poster tilted "Probing the role of Ferredoxins in Cyanobacterial Biotransformations", and
  • Malene Olsen from the Department of Food Science, University of Copenhagen, Danmark, for the poster tilted "Breeding of Nannochloropsis oceanica for increaased growth rates using random mutagenesis".
Awaiting your free antibody choice!

The book "Photosynthesis, solar energy for life" was awarded to Shaikh Mohd Kashif from Univeirsity of Gothenburg as the first prize in a Quiz about proteins, plant science and antibodies, conducted by Agrisera. 

Thank you to all the conference participants for interesting discussions, suggestions and ideas!
 
1. Agrisera's table at the conference.
2. Researchers from Finland and Germany outside of Agrisera's facilities during a visit.
3. Left to right: Malene Olsen (winner Best Poster Prize), Lenny Maliham-Yap (winner Best Poster Prize), Shaikh Mohd Kashif (winner Agrisera quiz), Joanna Porankiewicz-Asplund (Agrisera's conference representative and Technical Support Manager).

 
Read more 2023-06-21

Localization of plant clathrin using immunocytochemistry and Agrisera directly labelled anti-clathrin 1,2 antibodies

Material: Zea mays hybrid variety

Preparation
5 days-old germinating maize root tips were cut and fixed with 4% formaldehyde, 60 min, RT. Cell walls were digested with enzymes cellulase and pectinase in MES buffer for 90 min, RT. After washing with PBS, roots were squashed gently using a flat tip forceps to release the cells into PBS buffer. Cells were allowed to settle O/N at 4°C, followed by immunolocalization.

Immunocytochemistry (ICC)
Cells were permeabilized with 0.5% Triton X-100, 10 min, RT, followed by washing with PBS buffer before blocking with 5% fish gelatin-PBS, 30 min, RT.

Detection antibody: Cells were incubated with rabbit anti-clathrin 1,2 primary antibodies conjugated with DyLight®594 (AS10 690-DL594, Agrisera) for 3h/RT. Nuclei were stained with DAPI followed Fluoromount-G mounting (Southern Biotech).

Courtesy of Dr. Ferhan Ayaydin and Dr. Divya Teja Dondapati, Hungarian Centre of Excellence for Molecular Medicine, (HCEMM), Szeged, Hungary.
   Immunlofluorescent localization of clathrin on plant tissue
Upper panels show the Differential Interference Contrast (DIC) transmitted light image (gray) and Clathrin immunodetection (red). Lower panels represent DAPI labelled nuclei (cyan) and the merged image of Clathrin and DAPI.


Read more 2023-06-20

Agrisera at ICAR2023

Agrisera attended the 33rd International Conference on Arabidopsis Research (ICAR2023) in Chiba, Japan between the 5th and 8th of June, 2023.

A big thank you to everyone who came by Agrisera's booth to talk antibodies and plant science!

We would also like to give a big thank you the organizers and to our Japanese distributor Funakoshi, who accompanied us in the booth and invited us for a lovely meeting and dinner in Tokyo! Thank you to Ms. Ayuna Kisanuki, Mr. Akihiro Yoshida, Mr. Koichi Tanaka, Mrs. Sachie Kai and Ms. Aoi Sawada!

Agrisera's mascot and antibody expert Buddy was also present at the conference.
  
Agrisera's conference booth at ICAR2023.


Left to right: Akihiro (Funakoshi), Edith (Agrisera), Ayuna (Funakoshi), Koichi (Funakoshi).

Read more 2023-06-19

Immunofluorescent localization using Agrisera epitope tag antibodies

Take an advantage of Agrisera epitope tag antibodies in fluorescent immunolocalization of proteins under investigation

Immunofluorescent localization using Agrisera epitope tag antibodies
Various antibodies to epitope tags have been used for immunoifluorescent localization of target proteins in transfected HEK293 cells, including anti-HA (AS12 2220), anti-3xHis (AS20 4441) and anti-Myc (AS21 4529). Details of employed protocols are provided on the specific antibody product pages. 

Images courtesy of Courtesy of Dr. Anna K Överby, Molecular Infection Medicine Sweden (MIMS), Section of Virology. Department of Clinical Microbiology Umeå University, Sweden.

Read more 2023-06-14

Agrisera Western blot workshop for Centre for Research in Agricultural Genomics (CRAG)

On the 12th of June Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager, conducted an online workshop for researchers from the Centre for Research in Agricultural Genomics (CRAG), Spain. The title of the workshop was "Western blot – a technique possible to optimize".

The workshop was almost two hours long, including a 10 minutes break for the Agrisera Antibody and Western blot Quizzes and coffee. Questions submitted by attendees were answered, and many pitfalls of the Western blot technique were discussed, with specific experimental examples. 

Participants also had a chance to win Agrisera high titer secondary antibodies and 2 ECL reagents, in two quizzes, consisting of questions about antibodies and the Western blot technique.

Each participant of the workshop received a pdf of the presentation with links to additional information. The recording of the workshop is available on demand. 

If you are interested in this type of online workshop to be held at your institution, please contact us!
  Work flow in Western blot technique

Feedback from one of the participants: "Thank you very much for the seminar, I think was very interesting and enjoyable."

Read more 2023-06-14

Plant actin visualization using expansion microscopy with Agrisera anti-actin monoclonal antibodies

Sample: Maize protoplasts fixed in 4% paraformaldehyde in 1X PBS pH 7.4 for 1 hour and washed 3 times in 1XPBS buffer

Primary antibodies: Agrisera AS21 4615 actin monoclonal; clone 14H4G8;

Secondary antibodies: Agrisera Donkey anti-Mouse IgG (H&L), DyLight® 488 conjugated AS10 1201

Other reagents: 30% Bovine Serum Albumin solution, Sigma Aldrich Cat # A8577-50ML; Phosphate Buffered Saline 10X, Electron Microscopy Sciences Cat # 19342-10; Imaging spacer, Millipore Sigma Grace Bio-Labs SecureSeal™ imaging spacer Cat # GBL654008

Protocol:

  1. Block isolated protoplasts in a blocking buffer (2% BSA in 1x PBS) for 1 h at room temperature.
  2. Dilute the primary antibody to 1: 500 in a blocking buffer, mix well, and spin down at 150 x g for 3 min:
  3. Spin down protoplasts in a blocking buffer at 150 g for 1 -3 min and remove as much supernatant as possible using a pipette tip with a cut end (wide opening).
  4. Resuspend protoplasts in diluted antibody and incubate for 1 h at room temperature.
  5. Spin down protoplasts at 150 g for 1 min and remove as much supernatant as possible using a pipette tip with a cut end.
  6. Wash protoplasts in a blocking buffer 3 times for 5 minutes each. Spin and resuspend as described in step 5 between each washing step.
  7. Dilute the secondary antibody AS10 1201 to 1: 400, mix well, and spin down at 150 x g for 3 min:
  8. Incubate protoplasts in diluted antibody for 1 h at room temperature.
  9. Repeat the washing steps as described in 5-6.
  10. Resuspend a protoplast pellet in a small volume of PBS 1X, pH 7.4.
  11. Prepare microscopy slide: attach imaging spacer on top of the slide, peal off top adhesive membrane, add protoplast suspension on to the slide inside the spacer opening, cover with 22x22 mm cover glass No 1.5.
  12. Imaging: Zeiss Elyra 7 SIM

Courtesy of Dr. Anastasiya Klebanovych, Dr. Kirk Czymmek, Dr. Kevin Cox, Dr. Blake Meyers, at the Donald Danforth Plant Science Center, USA

  
 Plant actin visualization using Agrisera monoclonal antibodies AS21 4615

Read more 2023-06-09

Agrisera Prizes awarded during International Plant Proteomics Organization Conference

Agrisera supported the 5th International Plant Proteomics Organization (INPPO) conference, held between the 22nd and 25th of May 2023, in Thessaloniki, Greece. The conference focused on basic and applied plant proteomics research. 

The meeting covered the following topics:

  • Plant stress under climate crisis
  • Developmental proteomics
  • Signaling in plant interactions
  • Systems biology studies
  • Technical/methodological advances in proteomic analysis


The winners of Agrisera Best Poster Prizes, as well as a free antibody of their choice from the Agrisera catalog, were Dr. Jingjing Huang from Department of Plant Biotechnology and Bioinformatics, Ghent University and VIB Center for Plant Systems Biology,Belgium. and PhD student Siel Goethals from VIB-UGent Plant Systems Biology, Belgium

Awaiting your free antibody choices!

 Agrisera awards druing INPPO 2023

Read more 2023-06-02
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