Agrisera blog

Loading control antibodies are used to assess if the protein samples have been loaded equally across the gel, as well as transferred to the membrane. Such controls need to be ubiquitously and constitutively expressed proteins. Commonly used loading controls are the following proteins: actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and tubulin. 

Actin and tubulin are highly conserved proteins, which play essential roles in the plant cytoskeleton. Actin is involved in cellular processes, such as cell division, cell share maintenance and intracellular transport. 
Tubulin is a component of micotubules, which are critical for cell division, intracellular transport and maintaining cell structure. 
 
A protein that is chosen to be used as a loading control should remain stable across the applied experimental conditions. Please note that in certain stresses in plants, actin levels may change. 

Take an advantage of Agrisera's ongoing promotion for plant developmental biology antibodies, which are offered at a 20% discount until end of September, and use promotional code Dev20. 

• Polyclonal antibodies to plant actin (most published) AS13 2640 with a wide species range of di and monocotyl plants.
• Monoclonal/recombinant antibodies to plant actin (most published) AS21 4615 with a wide species range of di and monocotyl plants. 
• Polyclonal antibodies to: Tubulin alpha chain | Tubulin beta chain | Tubulin gamma chain  
• Monoclonal antibodies to: Tubulin alpha chain | Tubulin beta chain | Tubulin gamma chain
  
  
  Available for immunolocalization as well as Western blot techniques.

Agrisera supported the Portuguese Spanish Congress of Plant Biology 2023, which took place between the 9th and 12th of July in Braga, Portugal. Congress topics covered diverse fields, like the adaptation of plants, biotic and abiotic stresses, molecular mechanisms that control plant development and function, plant biotechnology and sustainable crop production.

The winners of Agrisera Best Poster Prizes, who will receive a free antibody of their choice from the Agrisera catalog, were:

• Dr. Fernando Evaristo Díaz‐Manzano from Universidad de Castilla‐La Mancha, Toledo, Spain for the poster "Bacillus spp. as a phytopathogenic biocontrol tool against the nematode Meloidogyne spp. in tomato"
  
• PhD student Ana Álvarez from Plant Physiology, Faculty of Biology, Department of Organisms and Systems Biology, University of Oviedo, Asturias, Spain and Institute of Biotechnology of Asturias (IUBA), University of Oviedo, Asturias, Spain, for the poster "Combined effect of heat and drought stress reveal specific responses in different plant species mediated by common and species‐specific mechanisms".

• Dr. Liudmyla Kozeko from M. G. Kholodny, Institute of Botany of NAS of Ukraine, Kyiv, Ukraine, currently Nature Research Centre, Vilnius, Lithuania, and
• Dr. Sónia Silva from University of Aveiro, Portugal.

• To study plant organelles such as chloroplasts, mitochondria, peroxisomes, and the endoplasmic reticulum.
  
  
• To study plant cell biology and development by visualizing cell division, differentiation and other cellular processes in real-time and on intact plant tissues.
  
  
• To monitor plant stress response, by fusing GFP to stress-responsive genes, changes in GFP fluorescence in response to various environmental stresses can be monitored.
  
  
• For investigation of plant-microbe (bacteria and fungi) interactions by tagging those microorganisms with GFP.
  
  
• For transgenic plant studies, where GFP is used as a marker in the generation and selection of transgenic plants.
  
  
• For environmental monitoring in ecological studies, GFP has been used as a biomarker to detect and monitor pollution, like algae.
  
  
• In pollination and seed dispersal studies.

Feedback from the participants:

"The feedback from your workshop was quite good our students really enjoyed it and said it was very practical and addressed real questions."

"Thank you very much for the prizes and the information of the workshop. As a starter to the western blot world, the knowledge that has been delivered from the workshop really helped me build up a general understanding of western blot as well as trouble shooting the potential problems. I love the FAQ part of each section as I hold the same questions many times."

"I would like to know whether you have a GFP antibody which works well to detect fusion proteins generated in plants? The GFP we used is eGFP from Aequorea victoria. We tried anti-GFP antibodies from other suppliers, but neither works well for our protein driven by native promoter."

Here is our answer: 

Yes, Agrisera offers both polyclonal and monoclonal antibodies to GFP (Green Fluorescent Protein), which detect fusions with plant proteins. Below are links to some of these products.

Anti-Venus polyclonal antibodies
for Western blot. 

Anti-GFP, polyclonal antibodies
for ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB).

Anti-GFP polyclonal antibodies
for Immunofluorescent localization in plant tissue and Western blot (WB).

Anti-GFP, monoclonal antibodies

Particular focus of the conference was given to plants and climate change, algal biology and bioenergy.

• Agrisera high-titer secondary antibodies, 1: 25 000 1h/RT (AS09 602)
• Agrisera outstanding ECL reagents, with mid picogram detection range (ECL Bright), and extreme low femtogram detection range (ECL SuperBright)