IKP

Tubulin beta chain (monoclonal antibody)

Product no: AS20 4484

AS20 4484  |  Clonality: Monoclonal  |  Host: Mouse |  Reactivity: Human, mouse, pig

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  • Data sheet
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  • Product Info
  • Immunogen: Beta-tubulin purified from porcine brain, UniProt: Q9H4B7
    Sub class: IgM
    Host: Mouse
    Clonality: Monoclonal
    Purity: Purified by precipitation and affinitychromatography in TBS. Contains 15 mM sodium azide.
    Format: Liquid
    Quantity: 100 g
    Storage: Store at 4C; Do not freeze. Do not exceed exipry date is provided on the tube. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunocytochemistry (ICC), Immunohistochemistry on frozen sections (IHC), Western blot (WB)
    Recommended dilution: 2-8 µg/ml  (ICC), 1 µg/ml (WB)
    Expected | apparent MW: 50,3 kDa
  • Reactivity
  • Confirmed reactivity: human, mouse, pig
    Predicted reactivity: Plants
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information (application): This antibody is recognizing N-terminal structural domain of beta tubulin.

    Recommended secondary antibody for Western blot is: goat anti mouse IgM AS16 3497

    Metal induced stress affected the expression of tubulin alpha and gamma, and that therefore, these proteins cannot be used as a loading control under that type of conditions. More information can be found here.
  • Background
  • Background: Tubulin beta (TUB) together with alpha tubulin is making up microtubules.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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AS10 681 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana, Medicago sativa

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AS16 3497 | Clonality: Polyclonal | Host: Goat | Reactivity: Mouse IgM
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