Tubulin beta chain
AS10 681 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana, Medicago sativa
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KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana tubulin beta-2/beta-3 chain P29512, beta-4 chain P24636(At5g44340), beta-5 chain P29513(At1g20010), beta-6 chain P29514(At5g12250), beta-7 chain P29515(At2g29550), beta-8 chain P29516(At5g23860), beta-9 chain P29517(At4g20890).
49 | 49 kDa (Arabidopsis thaliana)
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Tubulin beta localization in Arabidopsis thaliana epidermis cells. Tubulin beta localized to division plates visualized in green, nucleus with DAPI in blue. Plant material has been fixed in para-formaldehyde for 30 minutes. Tissue cleaning has been performed before immunolocalization. Primary antibodies: Agrisera anti-tubulin beta priamry antibody in dilution 1: 1000 and Agrisera goat anti-rabbit IgG DyLight® 488, secondary antibody in dilution 1: 2000. Scale bar – 10 µm.
(A) The 3 days old suspension cells of Arabidopsis thaliana have been fixed for 25 minutes with 2% FA, cell wall was partaillly digested with 0.2% Dricelase in MES buffer (pH 5.1) for 30 min at 37°C and the cells were dried on coated slide to omit PM extraction. Following by incubation for 45 minutes with Agrisera anti-tubulin beta chain primary antibody in a dilution of 1: 1000, washing once and 45 min incubation with a secondary goat anti-rabbit Alexa 555 antibody, in a dilution of 1:1000 dilution (Invitrogen). Cells were counterstained witn DAPI and visialized under epi/fluiresecnet microscope (B).
Courtesy Dr. Taras Pasternak, Freiburg University, Germany
Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.
Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 µg/well and a dilution of 1: 500.
Tubulin beta (TUB) together with alpha tubulin is making up microtubules.
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