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GFP | Green Fluorescence Protein

AS20 4443 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Green Fluorescence Protein (GFP)

GFP | Green Fluorescence Protein in the group Tag Antibodies / GFP/YFP/RFP/CFP at Agrisera AB (Antibodies for research) (AS20 4443)

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Product Information

Immunogen Recombinant GFP protein derived from Aequorea victoria, UniProt: P42212
Host

Rabbit

Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1: 5000 - 1 : 10 000 (WB)

Reactivity

Confirmed reactivity Recombinant GFP overexpressed in E.coli, from: Arabidopsis thaliana, Chlamydomonas reinhardii, Nicotiana tabacum

Application examples

Application examples
Western blot using rabbit anti-GFP antibodies

194 µg/well of total protein extracted freshly from tobacco with write exact buffer components (50mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 10% Glicerol, 1 mM DTT, 1X Pefablock) and denatured with SDS 2% and 0.1% Bromophenol blue (Laemmli buffer) at 95°C for 5 min.... were separated on 12 % SDS-PAGE and blotted 2hs to PVDF/nitrocellulose (pore size of um), using wet transfer. Blot was blocked with 5% milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 in TBS-T ON/4°C (with or without milk) with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:20 000 in for 1h/RT with agitation. The blot was washed as above and developed for 2 min with Agrisera ECLSuperBright with name and supplier. Exposure time was 60 seconds.

Courtesy of Delfina Gagliardi Instituto de Agrobiotecnología del Litoral (IAL), Argentina

Additional information

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Background

Background GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

Product citations

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Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items