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GFP | Green Fluorescence Protein

AS20 4443 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Green Fluorescence Protein

GFP | Green Fluorescence Protein in the group Tag Antibodies / GFP/YFP/RFP/CFP at Agrisera AB (Antibodies for research) (AS20 4443)

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Product Information

Immunogen KLH-conjugated peptide derived from GFP protein derived from Aequorea victoria, UniProt: P42212
Host

Rabbit

Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl, of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 10 000 (WB)

Reactivity

Confirmed reactivity Recombinant GFP overexpressed in E.coli, Nicotiana tabacum

Application examples

Additional information

Related products

Background

Background GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

Product citations

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