Green Fluorescent Protein (GFP) of the jellyfish Aequorea victoria, that lived on Earth for millions of years before being discovered, was first purified and characterized in 1962, by Osamu Shimomura, a Japanese organic chemist and marine biologist. GFP is excited by light in the blue/violet/ultraviolet portion of the spectrum, and emits light in the green portion (hence the name). The protein emits light by undergoing a process called photobleaching, where it absorbs light energy and then re-emits it as fluorescence. Tagging a target protein with GFP allows its visualization, and the detection of its transgenic expression in vivo, as well as its subcellular localization, cell visualization and investigation of protein-protein interactions. It can be used for cell tracing and tracking. This easily detectable fluorescence has made GFP an invaluable tool in various biological studies, enabling researchers to visualize and understand complex biological processes in real-time. Image: Rabbit polyclonal antibody to GFP, AS20 4443 used on roots of Arabidopsis thaliana. Agrisera's antibody collection to Green Fluorescent Protein (GFP) and its variants (EGFP, S65T-GFP, RFP like mCherry, RS-GFP, YFP and Venus) includes both, polyclonal and monoclonal antibodies with different labels, enzymatic and fluorescent, which detect both native and denatured GFP. | How is GFP used in plant science?
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