Agrisera blog

Over 300 new publications added to product information sheets

Agrisera's product information sheets contain the most up-to-date information about:

Just in the year 2022, we have added 305 selected citations to our product information sheets. 

New information is updated on a weekly basis, to provide you with the most up-to-date product information sheets. In case of any questions, you are always welcome to contact Agrisera Support

 Scientific references for Agrisera products

Read more 2023-02-24

Agrisera at INUPRAG 2023 meeting

Between the 21st and 23rd of February, Agrisera participated in the 3rd INUPRAG symposium, held in Umeå, Sweden. This is a meeting of researchers from three collaborating institutions, namely Umeå Plant Science Centre (Sweden), INRAE (France) and CRAG (Spain). 

150 researchers, 34 posters and wide range of research topics:
  • Plant cell wall properties and dynamics
  • Epigenetic regulation
  • Population genetics
  • Omics approaches
  • Plant nutrition
  • Development and Signaling
  • Response to mechanical stress
  • Adaptation to abiotic stress 

Agrisera contributed with The Best Poster Prize, awarded to Dr. Marti Quevedo, former post doc in Dr. Åsa Strand group at UPSC. The poster title was: "Chloroplast retrograde signals modulate chromatin dynamics during plant greening". 

Thank you for interesting discussions, suggestions and ideas!

 Agrisera at INUPRAG 2023 meeting

Read more 2023-02-24

Quantitate NifH enzyme in samples from a wide range of organisms

Nitrogenase NifH is involved in the biological fixation of atmospheric nitrogen to ammonia. Alternative protein names are Nitrogenase component II, Nitrogenase Fe protein, Nitrogenase reductase, and FeMoCo-nitrogenase.

Agrisera Global anti-NifH antibodies are reactivie to this enzyme from the following species, as confirmed experimentally: Anabaena PCC7120, Clostridium butyricum, Codakia orbicularis, Cylindrospermopsis raciborskii CS-505, Dolichospermum sp., Nostoc sp, Rhodopseudomonas palustris, Trichodesmium sp., nodules of Trifolium repens L., Vibrio natriegens ATCC 14048

Beyond this, reactivity is predicted to the following species, based on sequence homology: Azotobacter vinelandii (Gram-), Bradyrhizobium japonicum, Cyanobacteria, Cyanothece ATCC51142, Desulfotomaculum reducens (strain MI-1), Clostridium cellobioparum, Enterobacter, genera,  euryachaeotes, Klebsiella pneumonia, Magnetococcus sp., Methanobacterium thermoautotrophicum, Methanococcus maripaludis, Methylobacterium sp., Mesoorhizobium loti, Rhodopseudomonas palustris TIE-1 strain, alpha, gamma, beta proteobacteria, enterobacteria, low GC gram+, high GC gram+, able to fix atmoshperic nitrogen, Rhizobium meliloti

Confirmed and predicted reactivity is explained further here

By using NifH antibodies, combined with NifH protein standard it is possible to quantify the enzyme using quantitative Western blot, described here, and in this video tutorial.

Until end of March 2023, both products are offered with a 20% discount
  Quantification of NifH enzyme in samples from a wide range of organisms

Total Trichodesmium sp. protein extract (lanes 6-11, 80 pmol chlorophyll loaded) extracted with PEB (AS08 300), and NifH protein standard (lanes 1-5, 0.05, 0.1, 0.3 0.75 and 1.5 pmol standard loaded)

Read more 2023-02-17

Agrisera supported Molecular Biology of Plants 2023 meeting

The 36th conference Molecular Biology of Plants, organized by the Section Plant Physiology and Molecular Biology of the Germany Society for Plant Sciences took place the 6th-9th of February, North Rhine-Westphalia, Germany. The organizers were Prof. Dr. Ute Höcker (Köln), Prof. Dr. Andreas Meyer (Bonn), and Prof. Dr. Marcel Quint (Halle).

Agrisera supported the meeting with The Best Poster Prize, a quiz on antibodies and Western blot, as well as hard copies of the Agrisera Educational Posters. The Best Poster Prize with the title "Shade signals as essential components of host detection in the parasitic plant Cuscuta campestris" was awarded to Dr. Beatrix Enderle, a postdoc in Prof. Hiltbrunner Lab at the University of Freiburg, Germany.

The winner of Agrisera Antibody Quiz was PhD student Srijana Raj from the Martin Luther University of Halle-Wittenberg, Germany. She will receive Agrisera high-titer secondary antibodies, goat anti-rabbit IgG, HRP conjugated, to be used at 1: 25 000 1h/RT, and a set with two ECL reagents of mid picogram and extreme low femtogram detection.

Thank you all who participated!
  Awardee of Agrisera Best Poster Prize at MBP2023

Read more 2023-02-17

IncuBlocker - efficient way to shorten Western blot time

Agrisera IncuBlocker is an "all in one" set for fast Western blot processing. The whole process takes place in a single step, as blocking, as well as primary and secondary antibody binding, occur simultaneously, followed by a wash step with included buffer. Depending on the titer of the primary antibody, it can be re-used up to 5 times (not recommended for quantification work). This means that the processing time can be shortened considerably, to 1.5 hour. 

Add your primary antibody and use your choice of chemiluminescence or chromogenic method to visualize the reaction. 

The set is provided for primary antibodies developed in chicken, mouse and rabbit, and is recommended for antibodies to proteins of high and moderate expression.

How to know the expression levels of your target protein?
Check in the Protein Abundance Database.
 IncuBlocker application example

The example above shows how the signal with a primary antibody can be visualized using IncuBlocker. No washes in between, as blocking and primary and secondary antibody incubationsn are done in one step. 

Read more 2023-02-10

Awardees of Agrisera Prizes at UCMR Day 2023

On the 19th of January, the 14th UCMR Day 2023 took place at Umeå Centre for Microbial Research, Umeå University. The meeting had outstanding talks and poster presentations, giving the possibility to present specific projects in the form of elevator talks. 

There were 50 poster presentations at the conference. A jury selected the top three posters, which were awarded prizes sponsored by UCMR and Agrisera.

Congratulations to these three articulate and ambitious researchers!
1. Winner of the best poster reward: PhD student Sandra Holmberg
2. Second place, the best poster: Dr. Dale Corkery
3. Third place, the best poster: Tom Guest

The winners are awarded an antibody of their choice from the Agrisera catalog.

Congratulations and awaiting your free antibody choice!
  Agrisera supports UCMR Day 2023 at Umeå Univrersity

Read more 2023-02-10

Highly performing matching secondary antibody for Agrisera rabbit primary antibodies

Agrisera goat anti-rabbit IgG secondary antibodies, conjugated to horseradish peroxidase (HRP), perform in dilution > 1: 25 000 in Western blot and ELISA, which is supported by a long list of publications.

As this anti-rabbit IgG secondary antibody performs in high dilutions, it offers advantages to other secondary antibodies in terms of minimizing background signal, and the number of experiments it can be used for. This makes it very economical, and strengthens experimental reproducibility.

Additionally, this antibody is provided in lyophilized format, which makes shipment economical and environmentally friendly, as there is no need to include dry ice. Shipments are done at ambient temperature, with perserved antibody performance, which has been tested and evaluated over the last 20 years.

Until the end of 2023, this product is offered at a 10% discount using the discount code: Conj10.

Agrisera high titer goat anti-rabbit IgG secondary antibodies conjugated to HRP
To the product: 1 mg or trial size.

Read more 2023-02-03
 Agrisera blog


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