Agrisera blog

Simultaneous detection with two primary antibodies

Two primary antibodies incubated on the blot at the same time - save time in the lab

Western blot using anti-PetA antibodies

Thylakoid membranes (10 µg of total chlorophyll) extracted freshly from Hordeum vulgare leaves with 100 mM HEPES-KOH (pH 7.5), 0.3 M sorbitol, 2 mM EDTA, and 1mM MgCl2 and denatured with a Laemmli buffer at 80°C for 5 min were separated on 12% SDS-PAGE and blotted 1 h to nitrocellulose (pore size of 0.2 um), using semi-dry transfer. Blot was blocked with 4% milk for 2 h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:3000 (PC and PetA, simultaneous western blot detection for both antibodies at the same time) for 1 h/RT with agitation in PBS-T. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5 min in PBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25000 in for 1 h/RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to manufactures recommendations. Exposure time was 30 seconds. Simultaneous western blot detection can be applied if MW of detected proteins differs in min. 20 kDa.

Courtesy Dr. Anja Liszkay, CNRS, France

Read more 2019-09-28

Agrisera on Botanikertagung 2019, International Plant Science Conference

Agrisera at Botanikertagung 2019

Between 15.09-19.09 Agrisera participated in Botanikertagung, International Plant Science Conference, which is a biannual meeting of German plant and algal scientists. This year it was held at Rostock University, Germany.

An Agrisera quiz, where the participants got to test their weight determining skills by predicting the weight of the Agrisera chemiluminescent reagents/secondary antibody set, attracted a lot of attention, and the winner actually provided the exact answer!

Our third educational poster, Regulation of photosynthesis was so popular that we ran out of it. Additional copies will be sent by post. Thank you to all who came by the Agrisera table and talked with us about western blot and antibody production service or shared their experience with antibody work. We will hopefully meet again in two years in Bonn!
Read more 2019-09-19

Agrisera at the 9th Conference of the Polish Society of Experimental Plant Biology

Agrisera at PSEB conference Torun Poland

Between the 9th and the 12th of September, Agrisera participated in the 9th biennial Polish Society of Experimental Biology Conference hosted by the Faculty of Biology and Environmental Protection, at the Nicolaus Copernicus University in Toruń, Poland. The theme of the Conference was "New trends in plant reproduction and growth regulation". The Best Poster Prize was funded by Agrisera and awarded to Dawid Kubiak from the group of prof. Janusz Niedojadło (bottom left on the picture) from the Department of Cellular and Molecular Biology, Faculty of Biology and Environment Protection, Nicolaus Copernicus University, Toruń. The title of the poster was "Epigenetic and epitranscriptomic regulation of gene expression". 

On the third day of the conference, Dr. Joanna Porankiewicz Asplund from Agrisera, held a workshop named "Tips and tricks of antibody production and western blot - how to obtain good results?". Thank you to all who participated!
The winner of the Agrisera Kahoot interactive quiz, who received a secondary antibody ECL set, was: Aleksandra Rosińska (top left picture) from Dr. Krystyna Oracz labThe set contains two chemiluminescent detection reagents (mid picogram and extreme low femtogram range) and an Agrisera goat anti-rabbit HRP-conjugated secondary antibody of high titer (min. 1: 25 000 1h/RT). 

Conference participants could test their weight measuring skills in an Agrisera quiz, conducted during each day of a conference. The winner, who correctly determined the weight of our secondary antibody ECL set, was Magdalena Czołpińska from UAM in Poznań. 

Thank you to everyone who came to the Agrisera table, to talk antibodies, share your experience with western blot, tell us about your work or participate in the quiz.

Read more 2019-09-15

The Global Plant Events Calendar Video

The Global Plant Events Calendar Video

The Global Plant Events Calendar launched in the end of January this year, is already listing over 150 worldwide plant conferences, workshops and meetings. This calendar makes it much easier to obtain a good overview on what is happening and allow better planning of conference attendance and organization. Following this link you will find a short video, showing which features this platform has and how to use it.

We hope that it is going to be useful!
Read more 2019-09-05

Agrisera on ADAM8 conference in Lund

ADAM8 Agrisera prize

During last week ADAM8 conference, Agrisera award for The Best Poster was given to Kalyani Sanagavarapu, PhD Student from Lund University. ADAM8th is the Scandinavian Conference, Amyloid Diseases and Amyloid Mechanisms (ADAM 8) held this year in Lund, covering exciting developments in the research fields of amyloid structures, biochemistry and disease diagnostics and mechanisms. The awarded poster title was:

"On the role of phosphorylation of serine residues in aggregation mechanism of amyloid β-peptide"

Warmest congratulations from Agrisera to Kalyani Sanagavarapu and awaiting your product choice from Agrisera catalogue, which will be delivered to you for free. Agrisera provides a collection of unique antibodies, which support research on amyloid diseases.

Good luck in your research!
Agrisera Team
Read more 2019-09-02

Agrisera at SPPS2019 conference in Ume

Agrisera at SPPS2019

Agrisera participated in the biannual Scandinavian Plant Physiology Society conference, between 28.08-30.08, held in Umeå, where one of Agrisera offices is located. As a surprise for participants, Agrisera conducted a Kahoot! quiz with questions regarding SPPS, Umeå and Agrisera. The happy winner of a free antibody from the Agrisera collection was Dr. Saijaliisa Kangasjärvi from the University of Turku. Second and third prize was the latest book about photosynthesis: Photosynthesis Solar Energy for Life by Dr. Dmitry Shevela, Prof. Lars Olof Björn, Prof. Govindjee, available also in the Agrisera webshop. 

Thank you for all interesting discussions and ideas, it is always a great pleasure to interact with resaerchers from Scandinavia!
Read more 2019-09-01
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