Goat anti-Rabbit IgG (H&L), HRP conjugated - trial sample
AS09 602-trial | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

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Product Information
Purified Rabbit IgG, whole molecule.
Store liquid material at 2-8°C up to 6 month.
Reactivity
Application examples
Comparison of Agrisera secondary antibody sensitivity

10 μg of mitochondrial fraction from Arabidopsis thaliana (1,3) and Arabidopsis thaliana leaf extract (2,4) were separated on 10% gel and blotted on nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Filters where blocked (1.5h) in 5% milk in TBST (1X TBS, 0,1% Tween 20), incubated with 1: 1000 anti-COXII antibodies (2h in TBST) followed by incubation with 1: 10 000 secondary anti-rabbit (1h) HRP-coupled antibodies from Agrisera (left panel) and other manufacture (right panel) and visualized with standard ECL on Kodak autoradiography film for 5 s. Antibody in left panel detects target protein also in total cell extract (2) and can be used in higher dilution than applied 1: 10 000.
Agrisera goat anti-rabbit HRP conjugated antibody (AS09 602) can be used at following dilutions: 1: 50 000 -1: 90 000 (ELISA), 1 : 75 000 with enhanced ECL and 1: 25 000 with regular ECL (WB), 1: 500 -1: 5000 (IHC).
Additional information
Affinity purified antibody is > 95 % pure, according to SDS-PAGE.
No reactivity is observed to non-immunoglobulin rabbit serum.
Background
Goat anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
Product citations
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