Goat anti-Rabbit IgG (H&L), HRP conjugated - trial sample

AS09 602-trial | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

Product Information

Immunogen

Purified Rabbit IgG, whole molecule.

Host Goat

Clonality Polyclonal

Purity Affinity purified goat IgG

Format Liquid

Quantity 10 µl

Storage

Store liquid material at 2-8°C up to 6 month.

Tested applications ELISA (ELISA) , Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 50 000 -1 : 90 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1: 10 000 -1 : 50 000 (WB)

Reactivity

Confirmed reactivity Based on IEP, this antibody Reacts with: Rabbit IgG heavy chainslight chains on all Rabbit immunoglobulins

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot detection of PsaC using Agrisera primary and secondary antibodies

5 µg of total extract from (1) Hordeum vulgaretotal leaf, (2) Zea mays (3) Spinacia oleracea extracted with PEB (AS08 300) were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary anti-PsaC antibody (AS04 042) at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (goat anti-rabbit IgG horse radish peroxidase conjugated, AGRISERA) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.

Comparison of Agrisera secondary antibody sensitivity

comparison of secondary antibody sensitivity

10 μg of mitochondrial fraction from Arabidopsis thaliana (1,3) and Arabidopsis thaliana leaf extract (2,4) were separated on 10% gel and blotted on nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Filters where blocked (1.5h) in 5% milk in TBST (1X TBS, 0,1% Tween 20), incubated with 1: 1000 anti-COXII antibodies (2h in TBST) followed by incubation with 1: 10 000 secondary anti-rabbit (1h) HRP-coupled antibodies from Agrisera (left panel) and other manufacture (right panel) and visualized with standard ECL on Kodak autoradiography film for 5 s. Antibody in left panel detects target protein also in total cell extract (2) and can be used in higher dilution than applied 1: 10 000.

Agrisera goat anti-rabbit HRP conjugated antibody (AS09 602) can be used at following dilutions: 1: 50 000 -1: 90 000 (ELISA), 1 : 75 000 with enhanced ECL and 1: 25 000 with regular ECL (WB), 1: 500 -1: 5000 (IHC).

Additional information

Additional information Antibody is provided in: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1% BSA (w/v), Protease IgG free, 0.1 % (v/v) Kathon CG.

Affinity purified antibody is > 95 % pure, according to SDS-PAGE.

No reactivity is observed to non-immunoglobulin rabbit serum.

Related products

AS09 607 | Goat anti-rabbit IgG (H&L) AP conjugated

AS09 608 | Goat anti-rabbit IgG (H&L) biotin conjugated

AS10 668 | Goat anti-Rabbit IgG (H&L) HRP conjugated, min.cross-reactivity to bovine/Human/mouse IgG/serum