HA (rabbit polyclonal)

AS12 2220 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Human influenza hemagglutinin (HA) tagged proteins (strong cross reactivity between 25 -20 kDa in Arabidopsis thaliana wildtype)

Product Information

Immunogen

KLH-conjugated synthetic peptide: YPYDVPDYA

Host Rabbit

Clonality Polyclonal

Purity affinity purified serum in PBS, pH 7.4.

Format Lyophilized

Quantity 100 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB), immunoprecipitation (IP)

Recommended dilution 1 : 5000 (WB)

Expected | apparent MW

Depends on the MW of the protein which is HA-tagged

Reactivity

Confirmed reactivity HA-tag

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

application example

Western blot using anti-HA tag antibodies

10 µg of total protein extracted freshly from mutant HEK293 cells with RIPA buffer and denatured at 65°C for 5 min were separated on 15% SDS-PAGE and blotted overnight to PVDF (pore size of 0.4 um), using wet transfer. Blot was blocked with 5% milk or 2 h at RT and then incubated in the primary antibody at a dilution of 1: 5 000 in a rotator for 2.5 h in 5% milk. The membrane was then placed in a clean container and rinsed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:20 000 in for 1 h at RT with agitation. The blot was washed as above and developed with Agrisera ECLSuperBright. Exposure time was 30 seconds.

Courtesy Dr. Małgorzata Wessels, Department of Medical Biochemistry and Biophysics, Umeå University, Sweden

Additional information

Important note: there is a strong cross reactivity between 25 -20 kDa in Arabidopsis thaliana wildtype.

Background

Background HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors.

Product citations

Selected references Hwang et al. (2019) Arabidopsis ABF3 and ABF4 Transcription Factors Act with the NF-YC Complex to Regulate SOC1 Expression and Mediate Drought-Accelerated Flowering. Mol Plant. 2019 Apr 1;12(4):489-505. doi: 10.1016/j.molp.2019.01.002

All references:	Hwang et al. (2019) Arabidopsis ABF3 and ABF4 Transcription Factors Act with the NF-YC Complex to Regulate SOC1 Expression and Mediate Drought-Accelerated Flowering. Mol Plant. 2019 Apr 1;12(4):489-505. doi: 10.1016/j.molp.2019.01.002
Picture (footer):	application example 10 µg of total protein extracted freshly from mutant HEK293 cells with RIPA buffer and denatured at 65°C for 5 min were separated on 15% SDS-PAGE and blotted overnight to PVDF (pore size of 0.4 um), using wet transfer. Blot was blocked with 5% milk or 2 h at RT and then incubated in the primary antibody at a dilution of 1: 5 000 in a rotator for 2.5 h in 5% milk. The membrane was then placed in a clean container and rinsed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:20 000 in for 1 h at RT with agitation. The blot was washed as above and developed with Agrisera ECLSuperBright. Exposure time was 30 seconds. Courtesy Dr. Małgorzata Wessels, Department of Medical Biochemistry and Biophysics, Umeå University, Sweden
background:	HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors.
Confirmed reactivity:	HA-tag
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information (application):	Important note: there is a strong cross reactivity between 25 -20 kDa in Arabidopsis thaliana wildtype.
calculated \| apparent molecular mass [kDa]:	Depends on the MW of the protein which is HA-tagged
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide: YPYDVPDYA
Purity:	affinity purified serum in PBS, pH 7.4.
Quantity:	100 µg
recommended dilution:	1 : 5000 (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB), immunoprecipitation (IP)

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