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HA (polyclonal)

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AS12 2220
| Clonality: Polyclonal | Host: Rabbit | Reactivity:Human influenza hemagglutinin (HA) tagged proteins (strong cross reactivity between 25 -20 kDa in Arabidopsis thaliana wildtype)

HA (polyclonal) in the group Antibodies for Human/Animal  / Tag Antibodies / HA at Agrisera AB (Antibodies for research) (AS12 2220)

DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide: YPYDVPDYA

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 100 µg
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB), immunoprecipitation (IP)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

depends on the MW of the protein which is HA-tagged

Reactivity

Confirmed reactivity HA-tag
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

 
Western blot using anti-HA tag antibodies

10 µg of total protein extracted freshly from mutant HEK293 cells with RIPA buffer and denatured at 65°C for 5 min were separated on  15% SDS-PAGE  and blotted overnight to PVDF (pore size of 0.4 um), using wet transfer. Blot was blocked with 5% milk or 2 h at RT and then incubated in the primary antibody at a dilution of 1: 5 000 in a rotator for 2.5 h in 5% milk. The membrane was then placed in a clean container and rinsed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:20 000 in  for 1 h at RT with agitation. The blot was washed as above and developed with Agrisera ECLSuperBright. Exposure time was 30 seconds.


Courtesy Dr. Małgorzata Wessels, Department of Medical Biochemistry and Biophysics, Umeå University, Sweden

Additional information

Important note: there is a strong cross reactivity between 25 -20 kDa in Arabidopsis thaliana wildtype.

Related products

Related products AS18 4176 | Anti-HA, monoclonal antibodies
Other HA-tag antibodies.

Background

Background HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors.

Product citations

Selected references Hwang et al. (2019) Arabidopsis ABF3 and ABF4 Transcription Factors Act with the NF-YC Complex to Regulate SOC1 Expression and Mediate Drought-Accelerated Flowering. Mol Plant. 2019 Apr 1;12(4):489-505. doi: 10.1016/j.molp.2019.01.002

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