Depending on the target, antibody validation can be done in different ways. This is discussed by interviewees of an article published in August 2022 on Biocompare. The experts in the field of antibody development who contributed to the article were: Joanna Porankiewicz-Asplund, Ph.D., Technical Support Manager at Agrisera AB Eileen Gonzalez, Ph.D., Associate Product Manager for Antibody Applications at BioLegend Emily Cartwright, Senior Product Marketing Specialist for Antibodies at Bio-Techne You can access the article here.
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