How antibodies are validated - interviews with experts

Depending on the target, antibody validation can be done in different ways. This is discussed by interviewees of an article published in August 2022 on Biocompare. The experts in the field of antibody development who contributed to the article were:

Joanna Porankiewicz-Asplund, Ph.D., Technical Support Manager at Agrisera AB
Eileen Gonzalez, Ph.D., Associate Product Manager for Antibody Applications at BioLegend
Emily Cartwright, Senior Product Marketing Specialist for Antibodies at Bio-Techne

You can access the article here.

Antibody binding to a target

Technical tips

Technical tips

Latest

Calclulated and aparent molecular weight of detected protein is different, why?
2025-10-09 How to chose right loading control for Western blot?
2025-10-06 Calclulated and aparent molecular weight of detected protein is different, why?
2025-10-03 Is an antibody going to work in a technique I am planning to use it in?
2025-09-30 Antibody reactivity to recombinant protein,does not validate antibody specificity in endogenous sample
2025-08-20 Can aggregated antibody be still used?
2025-08-15 Blot Once, Probe Twice - when such approach can be beneficial?
2025-07-31 Why should glycerol not be added, to stabilize antibodies in serum?
2025-06-27 Can methanol in transfer buffer be replaced by a less toxic alternative?
2025-05-12 Will the precipitation of SDS in the stripping buffer affect antibody stripping efficiency?
2025-04-29

Archive

Login / RetailerClose

Forgot password?