showForm Rabbit Anti-AGO1 | argonaute 1, HRP conjugated antibodies

Name:
Phone:
E-mail:
Message

AGO1 | Argonaute 1 (40 ĩg, HRP-conjugated)

AS09 527-HRP  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Nicotiana benthamiana

AGO1 | Argonaute 1 (40 ĩg, HRP-conjugated)  in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / microRNA at Agrisera AB (Antibodies for research) (AS09 527-HRP)

DATA SHEET IN PDF

Qty: 
427
How to cite this product:
Product name, number (Agrisera, Sweden)

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379, TAIR: At1g48410.

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Liquid
Quantity 40 µg
Storage Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

116.4 | 130 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana benthamiana
Predicted reactivity Brassica pekinensis, Capsella rubella, Malus domestica, Pisum sativum, Ricinus communis, Vitis vinifera
Not reactive in

Chlamydomonas reinhardtii

Application examples

Additional information

Additional information

Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.

TCA acetone total protein precipitation method

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Related products

Related products

AS09 527 | Anti-AGO1 | argonaute 1, rabbit antibodies
AS09 527-ALP | Anti-AGO1 | argonaute 1 (40 µg, ALP-conjugated), rabbit antibodies
AS09 527B | Anti-AGO1 | argonaute 1 (40 µg, Biotin conjugated), rabbit antibodies
AS14 2776 | Anti-AGO1 | argonaute 1 (Chlamydomonas), rabbit antibodies
AS09 527P | AGO1 | argonaute 1 | Blocking peptide
AS13 3682 | Anti-AGO2 | argonaute 2, rabbit antibodies
AS09 617 | Anti-AGO4 argonaute 4, rabbit antibodies
AS10 671 | Anti-AGO5 | argonaute 5, rabbit antibodies
AS10 672 | Anti-AGO6 | argonaute 6, rabbit antibodies
AS10 673 | Anti-AGO9 | argonaute 9, rabbit antibodies
AS15 3071 | Anti-AGO10 | argonaute 10, rabbit antibodies

collection of antibodies to micro RNA

Plant protein extraction buffer

Secondary antibodies

Background

Background

AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

This antibody is directly conjugated to Horseradish Peroxidase (HRP).

Product citations

Related products: AGO1 | Argonaute 1 (40 ĩg, HRP-conjugated)

AS18 4248 |  Clonality: Polyclonal  |  Host: Rabbit  |  Re...
1018 €
AS09 527-ALP  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivi...
427 €
AS09 527B  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:...
427 €
AS09 527  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: ...
278 €
Buy 2 items of this product for 206 €/items
Buy 3 items of this product for 190 €/items