AGO1 | Argonaute 1 (40 µg, HRP-conjugated)

Name: AGO1 | Argonaute 1 (40 µg, HRP-conjugated)
SKU: AS09 527-HRP
Price: 464 EUR
Availability: InStock

AS09 527-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana benthamiana

AGO1 | Argonaute 1 (40 µg, HRP-conjugated) in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / plant RNA at Agrisera AB (Antibodies for research) (AS09 527-HRP)

AGO1 | Argonaute 1 (40 µg, HRP-conjugated)

Product Information

Immunogen

KLH-conjugated N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379, TAIR: At1g48410.

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.

Format Liquid

Quantity 40 µg

Storage Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 116.4 | 130 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana

Predicted reactivity Brassica pekinensis, Capsella rubella, Glycine max, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in

Chlamydomonas reinhardtii, Oryza sativa, Phaseolus vulgaris, Triticum aestivum, Zea mays

Application examples

Additional information

Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U.

TCA acetone total protein precipitation method

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.

Background

AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi).

This antibody is directly conjugated to Horseradish Peroxidase (HRP).

Product citations

immunogen:	KLH-conjugated N-terminal peptide of Arabidopsis thaliana AGO1 UniProt: O04379, TAIR: At1g48410.
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.
Format:	Liquid
Quantity:	40 µg
storage:	Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
tested applications:	Western blot (WB)
recommended dilution:	1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	116.4 \| 130 kDa
Confirmed reactivity:	Arabidopsis thaliana, Hyacinthus orientalis, Nicotiana benthamiana
predicted reactivity:	Brassica pekinensis, Capsella rubella, Glycine max, Malus domestica, Pisum sativum, Ricinus communis, Solanum tuberosum, Zea mays, Vitis vinifera Species of your interest not listed? Contact us
not reactive in:	Chlamydomonas reinhardtii, Oryza sativa, Phaseolus vulgaris, Triticum aestivum, Zea mays
additional information:	Antibody binds microRNA and tasiRNAs, preference for 21nt miRNAs with 5'U. TCA acetone total protein precipitation method
additional information (application):	AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. The AGO1 antibody is extremely specific to AGO1 and does not cross-react with other antibodies. The evidence is 1) the peptide to which it was raised is at the very N-terminus of the protein and is not present in other AGOs 2) aAGO1 does not cross react with the AGOs which are overexpressed (AGO2, AGO3, AGO4, AGO5, AGO6, AGO9) using a western blot.
background:	AGO1 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). This antibody is directly conjugated to Horseradish Peroxidase (HRP).