BirA (mutated/TurboID) - ALP conjugated 40 ĩg
AS20 4440-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: E. coli BirA - mutated/TurboID overexpressed in various organisms
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Immunogen Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Purity Affinity purified serum, in PBS pH 7.4
Format Liquid, conjugated to ALP.
Quantity 40 µg
Storage Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW Depends upon fustion partner
Confirmed reactivity BirA (mutated/TurboID)
Not reactive in No confirmed exceptions from predicted reactivity are currently known
Background Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.
Selected references To be added when available, antibody available in January 2021.
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