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BirA (mutated/TurboID) - ALP conjugated 40 µg

AS20 4440-ALP  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: E. coli BirA - mutated/TurboID overexpressed in various organisms

BirA (mutated/TurboID)  - ALP conjugated 40 µg in the group Tag Antibodies / aadA1/BAR/BC2/BirA/CBP/GAL4/GUS/LUC at Agrisera AB (Antibodies for research) (AS20 4440-ALP)



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Product Information

Immunogen Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Host

Rabbit

Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Format Liquid
Quantity 40 µg
Storage Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW Depends upon fustion partner

Reactivity

Confirmed reactivity BirA (mutated/TurboID)
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Related products

Background

Background Tagging a protein with TurboID  allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.

Product citations

Selected references To be added when available, antibody available in January 2021.
immunogen: Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
Host:

Rabbit

Clonality: Polyclonal
Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to ALP.
Format: Liquid
Quantity: 40 µg
storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
recommended dilution: 1 : 5000 (WB)
calculated | apparent molecular mass [kDa]: Depends upon fustion partner
Confirmed reactivity: BirA (mutated/TurboID)
not reactive in: No confirmed exceptions from predicted reactivity are currently known
background: Tagging a protein with TurboID  allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.
All references: To be added when available, antibody available in January 2021.

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