Anti-BirA (mutated/TurboID) - Biotin conjugated (40 µg)
Product no: AS20 4440B
AS20 4440B | Clonality: Polyclonal | Host: Rabbit | Reactivity: E. coli BirA - mutated/TurboID overexpressed in various organisms
442
€
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- Product Info
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Immunogen: Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag). Host: Rabbit
Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin. Format: Liquid Quantity: 40 µg Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Recommended dilution: 1 : 5000 (WB) Expected | apparent MW: Depends upon fusion partner - Reactivity
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Confirmed reactivity: BirA (mutated/TurboID) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Background
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Background: Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
AS20 4440 | Clonality: Polyclonal | Host: Rabbit | Reactivity: E. coli BirA - mutated/TurboID overexpressed in various organisms
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AS20 4440-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: E. coli BirA - mutated/TurboID overexpressed in various organisms
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AS20 4440-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: E. coli BirA - mutated/TurboID overexpressed in various organisms
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