BirA (mutated/TurboID) - Biotin conjugated 40 µg

Product no: AS20 4440B

AS20 4440B  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: E. coli BirA - mutated/TurboID overexpressed in various organisms

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  • Product Info
  • Immunogen: Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177). Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag).
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.
    Format: Liquid
    Quantity: 40 µg
    Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
    Recommended dilution: 1 : 5000 (WB)
    Expected | apparent MW: Depends upon fusion partner
  • Reactivity
  • Confirmed reactivity: BirA (mutated/TurboID)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Background
  • Background: Tagging a protein with TurboID  allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019). Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa. 
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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