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Anti-BiP | Lumenal-binding protein, HRP-conjugated (40 µg)
AS09 481-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. napus, C. sativus, R. sativa L. Tokinashi-daikon, S. oleracea, S. lycopersicum, S. tuberosum, T. aestivum, Z. mays, O. europaea, P. abies, P. patens, Ch. reinhardtii
- Product Info
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Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007 , BiP3 At1g09080 Q8H1B3
Host: Rabbit Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP. Format: Liquid Quantity: 40 µg Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Tested applications: ELISA (ELISA), Western blot (WB) Recommended dilution: 1 : 8000 (ELISA), 1 : 2000 (WB) Expected | apparent MW: 73.5 | 80 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Brassica napus, Chlamydomonas reinhardtiiCucumis sativus, Nicotiana benthamiana, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Picea abies, Physcomitrium patens, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zea mays Predicted reactivity: Arabis alpina, Capsella rubella, Capsicum annuum, Citrus clementina, Citrus sinsensis, Eucalyptus grandis, Glycine max, Hordeum vulgare, Isatis tincorina, Nicotiana tabacum, Oryza sativa, Prunus persica, Triticum aestivium. Picea sitcHensis, Populus trichocarpa, Ricinus comminus, Vitis vinifera
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information (application): Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel, This antibody has so far not worked in IP - Background
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Background: BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER).
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters CollectionMethod of isolation of plant ER
Courtesy of Dr. Masayoshi Maeshima, Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences Nagoya University Nagoya, Japan
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Accessories
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AS09 481 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. napus, Chara australis, C. reinhardtii, C. sativus, M. perniciosa, N. benthamiana, N. tabacum, R. sativa, L.Tokinashi-daikon, O. europaea, O. sativa, P. abies, P. patens, S. oleracea, S. lycopersicum, S. tuberosum, T. aestivum, Z. mays
AS08 327 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Zea mays | Cellular [compartment marker] of Golgi in immunolocalization and of COP1 in western blot