Sec21p | Gamma subunit, COP vesicles

AS08 327  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Arabidopsis thaliana, Zea mays | Cellular [compartment marker] of Golgi in immunolocalization and of COP1 in western blot

Sec21p | Gamma subunit, COP vesicles in the group Antibodies for Plant/Algal  / Membrane Transport System / Endomembrane system at Agrisera AB (Antibodies for research) (AS08 327)


Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information


GST fusion of a part of recombinant Sec21 of Arabidopsis thaliana Q0WW26, At4g34450

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunofluorescence (IF), Western blot (WB)
Recommended dilution 1 : 1000 (IF), 1 : 1000 (WB)
Expected | apparent MW

98 kDa


Confirmed reactivity Arabidopsis thaliana, Phaeodactylum tricornutum, Zea mays
Predicted reactivity

Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Coffea canephora, Eutrema salsugineum, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Medicago truncatula, Oryza sativa, Populus trichocarpaPrunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays,

Species of your interest not listed? Contact us
Not reactive in Nicotiana tabacum, Microsporidia sp.

Application examples

Application examples

Application example

Western blot using anti-Sec21p

50 µg of total protein from (1) Nicotiana tabacum protoplast total protein, (2) Arabidopsis thaliana protoplast soluble protein, (3) Arabidopsis thaliana protoplast total protein were separated on 10 % SDS-PAGE and blotted 2h to nitrocellulose (Semi-dry, 200mA). Filters were blocked over night with 5% low-fat milk powder in TBS and probed with anti-Sec21p antibodies (AS08 327, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP) in TBS-Tween. Signal was detected with chemiluminescent detection reagent, exposure time was 1 minute.

Protoplasts were extracted in 50mM Tris, 10 mM EDTA and Triton X100, 0.02%.


immunofluorescence using anti-Sec21 antibodies

Immunofluorescence labelling of rabbit anti-SEC21 (gamma subunit of COP vesicles; red) in 5-day-old root epidermal cells of Arabidopsis thaliana expressing ER-mGFP5-HDEL (ER marker; green). The antibody was diluted 1:1000 and the secondary antibody, donkey anti-rabbit CY5-coupled (Jackson ImmunoResearch) was diluted 1:300. The nuclei were stained with DAPI (blue).

Courtesy of Dr. Anna Gustavsson and Dr. Markus Grebe, Umeå Plant Science Centre, Sweden

Additional information

Additional information This product can be sold containing proclin if requested.

This antibody can be used as a Golgi marker in immunolocalization and as a marker of COP1 in Western blot.

References describing immunolocalization (IF) and (IG) studies:

Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36.
Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.

Related products

Related products

AS10 683 | Anti-HDEL | endoplasmic reticulum retention signa, (100 µg), monoclonal antibodies

AS10 683-25 | Anti-HDEL | endoplasmic reticulum retention signal, (25 µg), monoclonal antibodies

AS08 325 | Anti-Arf1 | ADP-ribosylation factor 1, Golgi marker, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies



Sec21p is a constituent of the COPI vesicle coatomer.

Product citations

Related products: Sec21p | Gamma subunit, COP vesicles

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS08 325  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity:&...
292 €
Buy 2 items of this product for 218.00 €/items
Buy 3 items of this product for 198.00 €/items
AS09 607 Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
199 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

194 €