Name:
Phone:
E-mail:
Address:


 


IAPP | Human IAPP (amylin) 1-37, specific for the native hormone having a disulphide-bridge between Cys2-Cys7

AS08 359  |  clonality: polyclonal  |  host: hen  |  reactivity: human

IAPP | Human IAPP (amylin) 1-37, specific for the native hormone having a disulphide-bridge between Cys2-Cys7  in the group Antibodies for Human/Animal  / Human Proteins / Amyloid disorders / Alzheimer's disease at Agrisera AB (Antibodies for research) (AS08 359)

DATA SHEET IN PDF

Qty: 
286
Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

Synthetic peptide corresponding to the human the 37 residue IAPP also known as amylin. The IAPP/amylin peptide contains a disulphide-bridge between Cys2-Cys7

Amino acid sequence: KCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTY (disulphide link between Cys2-Cys7)

Host Chicken
Clonality Polyclonal
Purity Total IgY
Format Lyophilized
Quantity 50 µl
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution

1:1000 (WB), 1:1000 (ELISA)

Expected | apparent MW

3.9 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity

Primates, mouse, rat, dog, seal, Chinese hamster

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Application examples

Application examples
Western blot using anti-IAPP chicken antibodies

Western blot analysis illustrating specificity of AS08 359 for the oxidized form of IAPP (partly aggregated peptide illustrated below)
• Peptide separated on 16% SDS-tricine PAGE under denaturing conditions +/-β-mercaptoethanol.
• Membrane: PVDF
• Transfer buffer, tris glycine, pH 9.5 containing 0.025% SDS
• Blocking buffer: 0.4% BSA in TBS, 0.3% Tween 20
• Antibody dilution : 1:1000
• Secondary antibody: anti-chicken (HRP)
• Detection: Enhanced Chemiluminescence 10 seconds


Western blot using anti-IAPP chicken antibodies


A- no reducing agent
B- reducing agent - 1 % betamercaptoethanol

ELISA using anti-IAPP chicken antibodies

ELISA

  • Nunc immunosorpt 96 well plate was coated with synthetic IAPP (amylin) 1-37, in PBS at peptide concentration corresponding to 2μg/ml.
  • Blocking buffer: 0.4% BSA in TBS, 0.3% Tween 20
  • Antibody dilution : 1:1000
  • Washing solution TBS containing 0.3% tween-20
  • Secondary antibody: anti-chicken (HRP)

Additional information

Antibody is specific for the native hormone having a disulphide-bridge between Cys2-Cys7.

Related products

Background

Background

Amylin, or Islet Amyloid Polypeptide (IAPP) P10997, is a 37-residue peptide hormone secreted by pancreatic beta-cells at the same time as insulin (in a roughly 1:100 amylin:insulin ratio). Islet, or insulinoma, almyloid polypeptide (IAPP, or amylin) is commonly found in pancreatic islets of patients suffering diabetes mellitus type 2, or harboring an insulinoma. While the association of amylin with the development of type 2 diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Recent results suggest that amylin, like the related beta-amyloid (Abeta) associated with Alzherimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the deleopment of type 2 diabetes.

Product citations

Related products: IAPP | Human IAPP (amylin) 1-37, specific for the native hormone having a disulphide-bridge between Cys2-Cys7

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €