IAPP | Human IAPP (amylin) 1-37, specific for the native hormone having a disulphide-bridge between Cys2-Cys7

Name: IAPP | Human IAPP (amylin) 1-37, specific for the native hormone having a disulphide-bridge between Cys2-Cys7
SKU: AS08 359
Price: 302 EUR
Availability: InStock

AS08 359 | clonality: polyclonal | host: hen | reactivity: human

IAPP | Human IAPP (amylin) 1-37, specific for the native hormone having a disulphide-bridge between Cys2-Cys7 in the group Antibodies Human Cell Biology / Neuroscience / Neurodegenerative diseases / Alzheimer's disease at Agrisera AB (Antibodies for research) (AS08 359)

Product Information

Immunogen Synthetic peptide corresponding to the human the 37 residue IAPP also known as amylin, The IAPP/amylin peptide contains a disulphide-bridge between Cys2-Cys7 Amino acid sequence: KCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTY (disulphide link between Cys2-Cys7)

Host Chicken

Clonality Polyclonal

Purity Purified,total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Format Lyophilized

Quantity 50 µl

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution

1:1000 (WB), 1:1000 (ELISA)

Expected | apparent MW 3,9 kDa

Reactivity

Confirmed reactivity Human

Predicted reactivity

Primates, mouse, rat, dog, seal, Chinese hamster

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Western blot analysis illustrating specificity of AS08 359 for the oxidized form of IAPP (partly aggregated peptide illustrated below)
• Peptide separated on 16% SDS-tricine PAGE under denaturing conditions +/-β-mercaptoethanol.
• Membrane: PVDF
• Transfer buffer, tris glycine, pH 9.5 containing 0.025% SDS
• Blocking buffer: 0.4% BSA in TBS, 0.3% Tween 20
• Antibody dilution : 1:1000
• Secondary antibody: anti-chicken (HRP)
• Detection: Enhanced Chemiluminescence 10 seconds

Western blot using anti-IAPP chicken antibodies

Western blot using anti-IAPP chicken antibodies

A- no reducing agent
B- reducing agent - 1 % betamercaptoethanol

ELISA using anti-IAPP chicken antibodies

ELISA

Nunc immunosorpt 96 well plate was coated with synthetic IAPP (amylin) 1-37, in PBS at peptide concentration corresponding to 2μg/ml.
Blocking buffer: 0.4% BSA in TBS, 0.3% Tween 20
Antibody dilution : 1:1000
Washing solution TBS containing 0.3% tween-20
Secondary antibody: anti-chicken (HRP)

Additional information

Antibody is specific for the native hormone having a disulphide-bridge between Cys2-Cys7

Background

Amylin, or Islet Amyloid Polypeptide (IAPP) P10997, is a 37-residue peptide hormone secreted by pancreatic beta-cells at the same time as insulin (in a roughly 1:100 amylin:insulin ratio). Islet, or insulinoma, almyloid polypeptide (IAPP, or amylin) is commonly found in pancreatic islets of patients suffering diabetes mellitus type 2, or harboring an insulinoma. While the association of amylin with the development of type 2 diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Recent results suggest that amylin, like the related beta-amyloid (Abeta) associated with Alzherimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the deleopment of type 2 diabetes.

Product citations

immunogen:	Synthetic peptide corresponding to the human the 37 residue IAPP also known as amylin, The IAPP/amylin peptide contains a disulphide-bridge between Cys2-Cys7 Amino acid sequence: KCNTATCATQRLANFLVHSSNNFGAILSSTNVGSNTY (disulphide link between Cys2-Cys7)
Host:	Chicken
Clonality:	Polyclonal
Purity:	Purified,total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)
recommended dilution:	1:1000 (WB), 1:1000 (ELISA)
calculated \| apparent molecular mass [kDa]:	3,9 kDa
Confirmed reactivity:	Human
predicted reactivity:	Primates, mouse, rat, dog, seal, Chinese hamster
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Western blot analysis illustrating specificity of AS08 359 for the oxidized form of IAPP (partly aggregated peptide illustrated below) • Peptide separated on 16% SDS-tricine PAGE under denaturing conditions +/-β-mercaptoethanol. • Membrane: PVDF • Transfer buffer, tris glycine, pH 9.5 containing 0.025% SDS • Blocking buffer: 0.4% BSA in TBS, 0.3% Tween 20 • Antibody dilution : 1:1000 • Secondary antibody: anti-chicken (HRP) • Detection: Enhanced Chemiluminescence 10 seconds A- no reducing agent B- reducing agent - 1 % betamercaptoethanol ELISA Nunc immunosorpt 96 well plate was coated with synthetic IAPP (amylin) 1-37, in PBS at peptide concentration corresponding to 2μg/ml. Blocking buffer: 0.4% BSA in TBS, 0.3% Tween 20 Antibody dilution : 1:1000 Washing solution TBS containing 0.3% tween-20 Secondary antibody: anti-chicken (HRP)
additional information (application):	Antibody is specific for the native hormone having a disulphide-bridge between Cys2-Cys7
background:	Amylin, or Islet Amyloid Polypeptide (IAPP) P10997, is a 37-residue peptide hormone secreted by pancreatic beta-cells at the same time as insulin (in a roughly 1:100 amylin:insulin ratio). Islet, or insulinoma, almyloid polypeptide (IAPP, or amylin) is commonly found in pancreatic islets of patients suffering diabetes mellitus type 2, or harboring an insulinoma. While the association of amylin with the development of type 2 diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Recent results suggest that amylin, like the related beta-amyloid (Abeta) associated with Alzherimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the deleopment of type 2 diabetes.

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