IAPP | Human IAPP (amylin) 1-37, specific for the native hormone having a disulphide-bridge between Cys2-Cys7
AS08 359 | clonality: polyclonal | host: hen | reactivity: human
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1:1000 (WB), 1:1000 (ELISA)
Primates, mouse, rat, dog, seal, Chinese hamster
Western blot analysis illustrating specificity of AS08 359 for the oxidized form of IAPP (partly aggregated peptide illustrated below)
• Peptide separated on 16% SDS-tricine PAGE under denaturing conditions +/-β-mercaptoethanol.
• Membrane: PVDF
• Transfer buffer, tris glycine, pH 9.5 containing 0.025% SDS
• Blocking buffer: 0.4% BSA in TBS, 0.3% Tween 20
• Antibody dilution : 1:1000
• Secondary antibody: anti-chicken (HRP)
• Detection: Enhanced Chemiluminescence 10 seconds
A- no reducing agent
B- reducing agent - 1 % betamercaptoethanol
- Nunc immunosorpt 96 well plate was coated with synthetic IAPP (amylin) 1-37, in PBS at peptide concentration corresponding to 2μg/ml.
- Blocking buffer: 0.4% BSA in TBS, 0.3% Tween 20
- Antibody dilution : 1:1000
- Washing solution TBS containing 0.3% tween-20
- Secondary antibody: anti-chicken (HRP)
Amylin, or Islet Amyloid Polypeptide (IAPP) P10997, is a 37-residue peptide hormone secreted by pancreatic beta-cells at the same time as insulin (in a roughly 1:100 amylin:insulin ratio). Islet, or insulinoma, almyloid polypeptide (IAPP, or amylin) is commonly found in pancreatic islets of patients suffering diabetes mellitus type 2, or harboring an insulinoma. While the association of amylin with the development of type 2 diabetes has been known for some time, a direct causative role for amylin has been harder to establish. Recent results suggest that amylin, like the related beta-amyloid (Abeta) associated with Alzherimer's disease, can induce apoptotic cell-death in particular cultured cells, an effect that may be relevant to the deleopment of type 2 diabetes.
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