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GLO1 | Glyoxalase I (clone 6F10)

AS21 4568 | Clonality: Monoclonal |  Host:  Rat | Reactivity: human, simian, mouse

GLO1 | Glyoxalase I (clone 6F10) in the group Antibodies for Human/Animal  / Human Proteins / Amyloid disorders / Alzheimer's disease at Agrisera AB (Antibodies for research) (AS21 4568)

DATA SHEET IN PDF

Qty: 
320
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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Recombinant, full length mouse GLO1 UniProt: Q9CPU0 fused to GST
Host Rat
Clonality

Monoclonal

Subclass/isotype IgG2b kappa
Purity Affinity purified by chromatography in PBS with 50 % glycerol, filter sterilized
Format

Liquid

Quantity 100 µg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 27 kDa (mouse, 29 kDa (human, simian)

Reactivity

Confirmed reactivity Human, simian, mouse
Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using anti-GLO1 (vertebrate) antibodies

Whole cell extracts of simian COS-1 (1), mouse L929 (2), human HeLa (3) were separated on SDS-PAGE and blotted to a PVDF membrane, followed by blocking in 5 % non-fat milk for 1h/RT. The primary antibody was incubated at 1: 1000 for 1 h/RT, followed by washes and incubation with a secondary anti-rat IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.

Immunofluorescence using anti-GLO1 (vertebrates) antibodies
Immunofluorescent staining of HeLa cells with anti-GLO1 antibodies. Cells were fixed with 4 % paraformaldehyde, permabilized with 0.2 % Triton X-100. The primary antibodies were used at 1: 500 and the secondary antibodies, goat anti-rat IgG, FITC conjugated were used at 1: 5000. 

Additional information

Related products

Background

Background Glyoxalase I (GLO1) is an enzyme that plays a role in the detoxification of methylglyoxal (MG), a side-product of glycolysis, via condensation with glutathione to produce S-lactoyl-glutathione. GLO1 is a zinc metalloenzyme whose crystal structure has been solved. The bacterial and yeast enzymes are monomeric while the mammalian one is homodimeric and its sequence is well conserved. GLO1 is found over-expressed in some tumors. GLO1 has also been suggested to be involved in anxiety diseases, autism, and Alzheimer’s disease.

Product citations

Selected references Jiang et al. (2018). Role of the Glyoxalase System in Alzheimer's Disease. J Alzheimers Dis. 2018;66(3):887-899. doi: 10.3233/JAD-180413. PMID: 30400091.
Hovatta et al. (2005) Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice. Nature. 2005 Dec 1;438(7068):662-6. doi: 10.1038/nature04250. Epub 2005 Oct 23. PMID: 16244648.
Junaid et al. (2004) Proteomic studies identified a single nucleotide polymorphism in glyoxalase I as autism susceptibility factor. Am J Med Genet A. 2004 Nov 15;131(1):11-7. doi: 10.1002/ajmg.a.30349. PMID: 15386471; PMCID: PMC1360505.

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