GLO1 | Glyoxalase I (clone 6F10)
AS21 4568 | Clonality: Monoclonal | Host: Rat | Reactivity: human, simian, mouse
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Product Information
Immunogen
Recombinant, full length mouse GLO1 UniProt: Q9CPU0 fused to GST
Host
Rat
Clonality
Monoclonal
Subclass/isotype
IgG2b kappa
Purity
Affinity purified by chromatography in PBS with 50 % glycerol, filter sterilized
Format
Liquid
Quantity
100 µg
Storage
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
27 kDa (mouse, 29 kDa (human, simian)
Reactivity
Confirmed reactivity
Human, simian, mouse
Predicted reactivity
Species of your interest not listed? Contact us
Application examples
Application examples
Whole cell extracts of simian COS-1 (1), mouse L929 (2), human HeLa (3) were separated on SDS-PAGE and blotted to a PVDF membrane, followed by blocking in 5 % non-fat milk for 1h/RT. The primary antibody was incubated at 1: 1000 for 1 h/RT, followed by washes and incubation with a secondary anti-rat IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.
Immunofluorescent staining of HeLa cells with anti-GLO1 antibodies. Cells were fixed with 4 % paraformaldehyde, permabilized with 0.2 % Triton X-100. The primary antibodies were used at 1: 500 and the secondary antibodies, goat anti-rat IgG, FITC conjugated were used at 1: 5000.
Whole cell extracts of simian COS-1 (1), mouse L929 (2), human HeLa (3) were separated on SDS-PAGE and blotted to a PVDF membrane, followed by blocking in 5 % non-fat milk for 1h/RT. The primary antibody was incubated at 1: 1000 for 1 h/RT, followed by washes and incubation with a secondary anti-rat IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.
Immunofluorescent staining of HeLa cells with anti-GLO1 antibodies. Cells were fixed with 4 % paraformaldehyde, permabilized with 0.2 % Triton X-100. The primary antibodies were used at 1: 500 and the secondary antibodies, goat anti-rat IgG, FITC conjugated were used at 1: 5000.
Additional information
Background
Background
Glyoxalase I (GLO1) is an enzyme that plays a role in the detoxification of methylglyoxal (MG), a side-product of glycolysis, via condensation with glutathione to produce S-lactoyl-glutathione. GLO1 is a zinc metalloenzyme whose crystal structure has been solved. The bacterial and yeast enzymes are monomeric while the mammalian one is homodimeric and its sequence is well conserved. GLO1 is found over-expressed in some tumors. GLO1 has also been suggested to be involved in anxiety diseases, autism, and Alzheimer’s disease.
Product citations
Selected references
Jiang et al. (2018). Role of the Glyoxalase System in Alzheimer's Disease. J Alzheimers Dis. 2018;66(3):887-899. doi: 10.3233/JAD-180413. PMID: 30400091.
Hovatta et al. (2005) Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice. Nature. 2005 Dec 1;438(7068):662-6. doi: 10.1038/nature04250. Epub 2005 Oct 23. PMID: 16244648.
Junaid et al. (2004) Proteomic studies identified a single nucleotide polymorphism in glyoxalase I as autism susceptibility factor. Am J Med Genet A. 2004 Nov 15;131(1):11-7. doi: 10.1002/ajmg.a.30349. PMID: 15386471; PMCID: PMC1360505.
Hovatta et al. (2005) Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice. Nature. 2005 Dec 1;438(7068):662-6. doi: 10.1038/nature04250. Epub 2005 Oct 23. PMID: 16244648.
Junaid et al. (2004) Proteomic studies identified a single nucleotide polymorphism in glyoxalase I as autism susceptibility factor. Am J Med Genet A. 2004 Nov 15;131(1):11-7. doi: 10.1002/ajmg.a.30349. PMID: 15386471; PMCID: PMC1360505.
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