GLO1 | Glyoxalase I (clone 6F10)
AS21 4568 | Clonality: Monoclonal | Host: Rat | Reactivity: human, simian, mouse
|Data sheet||Product citations||Protocols||Add review|
Whole cell extracts of simian COS-1 (1), mouse L929 (2), human HeLa (3) were separated on SDS-PAGE and blotted to a PVDF membrane, followed by blocking in 5 % non-fat milk for 1h/RT. The primary antibody was incubated at 1: 1000 for 1 h/RT, followed by washes and incubation with a secondary anti-rat IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.
Immunofluorescent staining of HeLa cells with anti-GLO1 antibodies. Cells were fixed with 4 % paraformaldehyde, permabilized with 0.2 % Triton X-100. The primary antibodies were used at 1: 500 and the secondary antibodies, goat anti-rat IgG, FITC conjugated were used at 1: 5000.
Hovatta et al. (2005) Glyoxalase 1 and glutathione reductase 1 regulate anxiety in mice. Nature. 2005 Dec 1;438(7068):662-6. doi: 10.1038/nature04250. Epub 2005 Oct 23. PMID: 16244648.
Junaid et al. (2004) Proteomic studies identified a single nucleotide polymorphism in glyoxalase I as autism susceptibility factor. Am J Med Genet A. 2004 Nov 15;131(1):11-7. doi: 10.1002/ajmg.a.30349. PMID: 15386471; PMCID: PMC1360505.
Related products: GLO1 | Glyoxalase I (clone 6F10)
This product can b...