mAB-O | Mouse anti-human Abeta protein (3-10) region, oligomer-specific
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|Recommended dilution||10 ug/ml (IL), 1-2 ug/ml (Dot), 1 ug/ml (ELISA capture)|
|Expected | apparent MW||
|Predicted reactivity||Mouse, Bovine, Chicken, Dog, Porcine, Rabbit|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
Immunolocalization: human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.
|Selected references||Brännström et al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.|
Dot blot reaction of the binding capacity of mAB-O to fibrils, monomers and oligomers. Equal amounts of each sample were spotted on a nitrocellulose membrane and then dried. The membrane was blocked with 5% non-fat milk before incubated for 1 h with anti-mAB-O (25nM) and then with secondary antibody, anti-mouse HRP-conjugated (1:1500). The membrane was washed with PBS containing 0.25% Tween-20 before detection using ECL prime (GE Healthcare).
IHC used to illustrate the lack of binding of mAB-O to plaques. Tissue sections from the human AD hippocampus were de-waxed and rehydrated in ethanol and then incubated with AS08 357 (A) and mAB-O(B) at RT for 1h. The immunoreactivity was detected with the anti-mouse Peroxidase Reagent Kit (ImmPRESS, Vector Laboratories, Inc.) and then developed using the ImmPACT AEC Peroxidase Substrate kit (Vector Laboratories, Inc.).
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