Name:
Phone:
E-mail:
Address:


 


Amyloid beta oligomer-specific monoclonal antibody (OMAB), Biotinylated

AS10 932B | Clonality: Monoclonal | Host: Mouse | Reactivity: amyloid beta

Amyloid beta oligomer-specific monoclonal antibody (OMAB), Biotinylated in the group Antibodies for Human/Animal  / Human Proteins / Amyloid disorders / Alzheimer's disease at Agrisera AB (Antibodies for research) (AS10 932B)

DATA SHEET IN PDF

Qty: 
588
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen partly aggregated, recombinant peptide corresponding to the human Abeta (1-40). Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V
Host Mouse
Clonality Monoclonal
Subclass/isotype IgM
Purity Affinity purified
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 100 µl of sterile water.
Storage Store lyophilized/reconstituted at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunohistochemistry (IHC)
Recommended dilution Coating antibody at 2 µg/ml (ELISA), 1 : 500 (IHC)
Expected | apparent MW 4.5 kDa

Reactivity

Confirmed reactivity Human Abeta oligomers only
Predicted reactivity Rat
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide.

Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.

Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.

OMAB antibody is a versatile tool within research of Alzheimer’s disease. A sandwhich ELISA illustrates its potential regarding its high selectivity towards Aβ oligomers.

Related products

Related products

AS13 2716 | mAB-M | human Abeta protein (3-10) region, oligomer-specific, mouse monoclonal antibodies
AS13 2715 | mAB-O | human Abeta protein (3-10) region, oligomer specific, mouse monoclonal antibodies

Agrisera matching secondary antibody: Goat anti-mouse IgM (µ chain), HRP conjugated, min. cross-reactivity to human IgG/serum, AS10 969

Secondary antibodies

Background

Background

Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.

Product citations

Selected references Richman et al. (2013). In Vitro and Mechanistic Studies of an Anti-Amyloidogenic Self-Assembled Cyclic D,L-#-Peptide Architecture. J. Americal Chemical Societ, Jan 19.
Lindhagen-Persson et al. (2010). Amyloid-β Oligomer Specificity Mediated by the IgM Isotype – Implications for a Specific Protective Mechanism Exerted by Endogenous Auto-Antibodies. PLoS ONE.

Related products: Amyloid beta oligomer-specific monoclonal antibody (OMAB), Biotinylated

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 25 €
AS10 932 | Clonality: monoclonal | Host: Mouse | Reactivity: amyloid beta
588 €
AS13 2716   | Clonality: monoclonal  |  Host: Mouse  |  Reactiv...
597 €
AS09 627 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Mouse IgG (H&L...
199 €