Amyloid beta oligomer-specific monoclonal antibody (OMAB), Biotinylated

Name: Amyloid beta oligomer-specific monoclonal antibody (OMAB), Biotinylated
SKU: AS10 932B
Price: 609 EUR
Availability: InStock

AS10 932B | Clonality: Monoclonal | Host: Mouse | Reactivity: amyloid beta

Amyloid beta oligomer-specific monoclonal antibody (OMAB), Biotinylated in the group Antibodies Human Cell Biology / Neuroscience / Neurodegenerative diseases / Alzheimer's disease at Agrisera AB (Antibodies for research) (AS10 932B)

Product Information

Immunogen partly aggregated, recombinant peptide corresponding to the human Abeta (1-40), Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V

Host Mouse

Clonality Monoclonal

Subclass/isotype IgM

Purity Affinity purified in PBS pH 7.4.

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunohistochemistry (IHC)

Recommended dilution Coating antibody at 2 µg/ml (ELISA), 1 : 500 (IHC)

Expected | apparent MW 4,5 kDa

Reactivity

Confirmed reactivity Human Abeta oligomers only

Predicted reactivity Rat

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide.

Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.

Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.

OMAB antibody is a versatile tool within research of Alzheimer’s disease, A sandwhich ELISA illustrates its potential regarding its high selectivity towards A? oligomers

Background

Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.

Product citations

Selected references Richman et al. (2013). In Vitro and Mechanistic Studies of an Anti-Amyloidogenic Self-Assembled Cyclic D,L-#-Peptide Architecture. J. Americal Chemical Societ, Jan 19.
Lindhagen-Persson et al. (2010). Amyloid-β Oligomer Specificity Mediated by the IgM Isotype – Implications for a Specific Protective Mechanism Exerted by Endogenous Auto-Antibodies. PLoS ONE.

immunogen:	partly aggregated, recombinant peptide corresponding to the human Abeta (1-40), Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V
Reconstitution:	For reconstitution add 100 µl of sterile water
Sub class:	IgM
Host:	Mouse
Clonality:	Monoclonal
Purity:	Affinity purified in PBS pH 7.4.
Format:	Lyophilized
Quantity:	50 µg
storage:	Store lyophilized/reconstituted at 4°C. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunohistochemistry (IHC)
recommended dilution:	Coating antibody at 2 µg/ml (ELISA), 1 : 500 (IHC)
calculated \| apparent molecular mass [kDa]:	4,5 kDa
Confirmed reactivity:	Human Abeta oligomers only
predicted reactivity:	Rat
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide. Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min. Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.
additional information (application):	OMAB antibody is a versatile tool within research of Alzheimer’s disease, A sandwhich ELISA illustrates its potential regarding its high selectivity towards A? oligomers
background:	Soluble oligomeric assemblies of the Amyloid-β peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Aβ-assemblies constitute a very interesting therapeutic target. Identification of Aβ-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Aβ-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.
All references:	Richman et al. (2013). In Vitro and Mechanistic Studies of an Anti-Amyloidogenic Self-Assembled Cyclic D,L-#-Peptide Architecture. J. Americal Chemical Societ, Jan 19. Lindhagen-Persson et al. (2010). Amyloid-β Oligomer Specificity Mediated by the IgM Isotype – Implications for a Specific Protective Mechanism Exerted by Endogenous Auto-Antibodies. PLoS ONE.