2 | Photosynthesis Tool Kit - quantitation
AS04 051 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants, algae, cyanobacteria
DATASHEET IN PDF| Datasheet | Product citations | Protocols | Add review |
Product Information
KLH-conjugated syntetic peptides for respective antibodies, see product info sheets
Reactivity
Application examples
Additional information
|
Product number: |
Product name: |
Reconstitution: |
Recommended dilution: |
|
AS03 037 |
Rabbit Anti-RbcL Global antibody |
For reconstitution see lable on respective tube. |
1:5000-10 000 with ECL |
|
AS10 939 |
Rabbit Anti-PsaC Global antibody |
For reconstitution see lable on respective tube. |
1:1000 with ECL |
|
AS05 084 |
Rabbit Anti-PsbA Global antibody |
For reconstitution see lable on respective tube. |
1:10 000 with ECL |
* All primary antibodies in this kit are raised in rabbits.
Product information - Protein standards:
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Product number: |
Product name: |
Concentration: |
Size: |
Western Blot – Positive Control: |
|
AS01 016S |
PsbA * |
0.25 pmol/ μl |
41.5 kDa# |
To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a PsbA signal in this range. A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control. |
|
AS01 017S |
RbcL * |
0.15 pmol/ μl |
52.7 kDa |
To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a RbcL signal in this range. A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control. |
|
AS04 042S |
PsaC * |
0.15 pmol/μl |
11.5 kDa# |
To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 µl). For most applications a sample load of 0.2 µg of chlorophyll will give a PsaC signal in this range. A 2 ul load is optimal for most chemiluminescent detection systems as a positive control. |
*These proteins are larger than a respective native protein due to the addition of His-tag
* For reconstitution of standards see lable on respective tube.
Product information - Secondary antibody:
AS09 602-trial Goat anti-Rabbit IgG (H&L), HRP conjugated, 50 µl (2x25 µl)
Product information - ECLreagent:
AS16 ECL-N-10 AgriseraECL Bright (10 ml trial pack)
Educational information about Quantitative western blot can be found here: detailed method description, video tutorial
References: Brown et al. (2007). Resource dynamics during infection of Micromonas pusilla by virus MpV-SP1. Environmental Microbiology 9(11): 2720-2727. Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.
Background
This kit allows quantitation of major photosynthetic proteins: Rubisco, PsaC (PSI) and PsbA (PSII) as well as determination of PSI/PSII ratios using the western blot technique.
The kit contains following global antibodies, protein standards and secondary antibody:
Rabbit anti-RbcL (Form I and Form II). Product number: AS03 037
Rabbit anti-PsaC. Product number: AS10 939
Rabbit anti-PsbA Product number: AS05 084
Rubisco protein standard. Product number: AS01 017S
PsaC protein standard. Product number: AS04 042S
PsbA protein standard. Product number: AS01 016S
Goat anti-Rabbit IgG (H&L), HRP conjugated (50 µl), Product number: AS09 602-trial
AgriseraECL Bright (10 ml trial pack) ,AS16 ECL-N-10
Product citations
MacKenzie et al (2005). Large reallocations of carbon, nitrogen and photosynthetic reductant among phycobilisomes, photosystems and Rubisco during light acclimation in Synechococcus elongatus are constrained in cells under low environmental inorganic carbon. Arch of Microbiol. 183: 190 - 202.
Bouchard et al. (2006) UVB effects on the photosystem II-D1 protein of phytoplankton and natural phytoplankton communities. Photochem and Photobiol 82: 936-951.
Morash et al. (2007) Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Can J. of Bot. 85: 476-483(8)
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