Photosynthesis Tool Kit - quantitation
AS04 051 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants, algae, cyanobacteria
This discounted antibody set contains the following high titer, well published antibodies: anti-RbcL, anti-PsaC (PSI), anti-PsbA (PSII), and respective protein standards RbcL, PsaC, PsbA for quantification and determination of PSI/PSII ratio. The set is suitable for Western blot using samples from a wide range of species making this product suitable for teaching labs or mutant screening.
The set contains also high titer matching secondary antibody (AS09 602) and chemiluminescent detection reagent of mid picogram range.
DATA SHEET IN PDF| Data sheet | Product citations | Protocols | Add review |
This kit allows quantitation of major photosynthetic proteins: Rubisco, PsaC (PSI) and PsbA (PSII) as well as determination of PSI/PSII ratios using the western blot technique.
The kit contains following global antibodies, protein standards and secondary antibody:
Rabbit anti-RbcL (Form I). Product number: AS03 037
Rabbit anti-PsaC. Product number: AS10 939
Rabbit anti-PsbA Product number: AS05 084
Rubisco protein standard. Product number: AS01 017S
PsaC protein standard. Product number: AS04 042S
PsbA protein standard. Product number: AS01 016S
Goat anti-Rabbit IgG (H&L), HRP conjugated (5 x 10 µl), Product number: AS09 602-trial
AgriseraECL Bright (10 ml trial pack), Product number: AS16 ECL-N-10
Product Information
KLH-conjugated syntetic peptides for respective antibodies, see product info sheets
Reactivity
Application examples
Additional information
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Product number: |
Product name: |
Reconstitution: |
Recommended dilution: |
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Rabbit Anti-RbcL Global antibody |
For reconstitution see lable on respective tube. |
1:5000-10 000 with ECL |
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|
Rabbit Anti-PsaC Global antibody |
For reconstitution see lable on respective tube. |
1:1000 with ECL |
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|
Rabbit Anti-PsbA Global antibody |
For reconstitution see lable on respective tube. |
1:10 000 with ECL |
* All primary antibodies in this kit are raised in rabbits.
Product information - Protein standards:
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Product number: |
Product name: |
Concentration: |
Size: |
Western Blot – Positive Control: |
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PsbA * |
0.25 pmol/ μl |
41.5 kDa# |
To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a PsbA signal in this range. A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control. |
|
|
RbcL * |
0.15 pmol/ μl |
52.7 kDa |
To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a RbcL signal in this range. A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control. |
|
|
PsaC * |
0.15 pmol/μl |
11.5 kDa# |
To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 µl). For most applications a sample load of 0.2 µg of chlorophyll will give a PsaC signal in this range. A 2 ul load is optimal for most chemiluminescent detection systems as a positive control. |
*These proteins are larger than a respective native protein due to the addition of His-tag
* For reconstitution of standards see lable on respective tube.
Product information - Secondary antibody:
AS09 602-trial Goat anti-Rabbit IgG (H&L), HRP conjugated, 50 µl (2x25 µl)
Product information - ECLreagent:
AS16 ECL-N-10 AgriseraECL Bright (10 ml trial pack)
Educational information about Quantitative western blot can be found here: detailed method description, video tutorial
References:
Brown et al. (2007). Resource dynamics during infection of Microm
onas pusilla by virus MpV-SP1. Environmental Microbiology 9(11): 2720-2727. Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.
Background
Product citations
Morash et al. (2007) Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Can J. of Bot. 85: 476-483(8)
Bouchard et al. (2006) UVB effects on the photosystem II-D1 protein of phytoplankton and natural phytoplankton communities. Photochem and Photobiol 82: 936-951.
MacKenzie et al (2005). Large reallocations of carbon, nitrogen and photosynthetic reductant among phycobilisomes, photosystems and Rubisco during light acclimation in Synechococcus elongatus are constrained in cells under low environmental inorganic carbon. Arch of Microbiol. 183: 190 - 202.
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