Photosynthesis Tool Kit - quantitation
This discounted antibody set contains the following high titer, well published antibodies and is suitable for Western blot using samples from a wide range of species making. This product suitable for teaching labs or mutant screening.
Benefits of using this antibody set

Data sheet | Product citations | Add review |
The kit contains following products:
AS03 037, Rabbit anti-RbcL (Form I) (50 µl)
AS10 939, Rabbit anti-PsaC (50 µl)
AS05 084, Rabbit anti-PsbA (50 µl)
AS01 017S, Rubisco protein standard (100 µl)
AS04 042S, PsaC protein standard (100 µl)
AS01 016S, PsbA protein standard (100 µl)
AS09 602-trial, Goat anti-Rabbit IgG (H&L), HRP conjugated (2 x 10 µl)
AS16 ECL-N-10, AgriseraECL Bright (10 ml trial pack)
Product Information
Reactivity
Application examples
Additional information
Product number: |
Product name: |
Reconstitution: |
Recommended dilution: |
Rabbit Anti-RbcL Global antibody |
For reconstitution see lable on respective tube. |
1:5000-10 000 with ECL |
|
Rabbit Anti-PsaC Global antibody |
For reconstitution see lable on respective tube. |
1:1000 with ECL |
|
Rabbit Anti-PsbA Global antibody |
For reconstitution see lable on respective tube. |
1:10 000 with ECL |
* All primary antibodies in this kit are raised in rabbits.
Product information - Protein standards:
Product number: |
Product name: |
Concentration: |
Size: |
Western Blot – Positive Control: |
PsbA * |
0.25 pmol/ μl |
41.5 kDa# |
To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a PsbA signal in this range. A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control. |
|
RbcL * |
0.15 pmol/ μl |
52.7 kDa |
To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a RbcL signal in this range. A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control. |
|
PsaC * |
0.15 pmol/μl |
11.5 kDa# |
To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 µl). For most applications a sample load of 0.2 µg of chlorophyll will give a PsaC signal in this range. A 2 ul load is optimal for most chemiluminescent detection systems as a positive control. |
*These proteins are larger than a respective native protein due to the addition of His-tag
* For reconstitution of standards see lable on respective tube.
Product information - Secondary antibody:
AS09 602-trial Goat anti-Rabbit IgG (H&L), HRP conjugated, 20 µl (2x10 µl)
Product information - ECLreagent:
AS16 ECL-N-10 AgriseraECL Bright (10 ml trial pack)
Educational information about Quantitative western blot can be found here: detailed method description, video tutorial
References:
Brown et al. (2007). Resource dynamics during infection of Microm
onas pusilla by virus MpV-SP1. Environmental Microbiology 9(11): 2720-2727. Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.