IKP

Photosynthesis Tool Kit - quantitation

Product no: AS04 051set
AS04 051set | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants, algae, cyanobacteria

This discounted antibody set contains the following high titer, well published antibodies and is suitable for Western blot using samples from a wide range of species making. This product suitable for teaching labs or mutant screening.

Benefits of using this antibody set


1025
Add to cart
Delivery 3-6 business days
Delivery:  3-6 business days
  • Data sheet

  • The kit contains following products:

    AS03 037, Rabbit anti-RbcL (Form I) (50 µl)
    AS10 939, Rabbit anti-PsaC (50 µl)
    AS05 084, Rabbit anti-PsbA (50 µl)
    AS01 017S, Rubisco protein standard (100 µl)
    AS04 042S, PsaC protein standard (100 µl)
    AS01 016S, PsbA protein standard (100 µl)
    AS09 602-trial, Goat anti-Rabbit IgG (H&L), HRP conjugated (2 x 10 µl)
    AS16 ECL-N-10, AgriseraECL Bright (10 ml trial pack)
    • Product Info
    • Immunogen: KLH-conjugated syntetic peptides for respective antibodies, see product info sheets
      Host: Rabbit, Secondary antibody: Goat,
      Clonality: Polyclonal
      Quantity: 50 l of respective antibody, 100 l of each protein standard, 2 x 10 l of secondary antibody, 10 ml of ECL reagent,
      Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
      Tested applications: western blot (WB)
    • Reactivity
    • Confirmed reactivity: Algae, Cyanobacteria, Higher plants
    • Additional Information
    • Additional information: Product information - Primary antibodies:

      Product number:

      Product name:

      Reconstitution:

      Recommended dilution:

      AS03 037

      Rabbit Anti-RbcL

      Global antibody

      For reconstitution see lable on respective tube.

      1:5000-10 000 with ECL

      AS10 939

      Rabbit Anti-PsaC

      Global antibody

      For reconstitution see lable on respective tube.

      1:1000 with ECL

      AS05 084

      Rabbit Anti-PsbA

      Global antibody

      For reconstitution see lable on respective tube.

      1:10 000 with ECL


      * All primary antibodies in this kit are raised in rabbits.


      Product information - Protein standards:

      Product number:

      Product name:

      Concentration:

      Size:

      Western Blot – Positive Control:

      AS01 016S

      PsbA *

      0.25 pmol/ μl

      41.5 kDa#

      To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a PsbA signal in this range.

      A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control.

      AS01 017S

      RbcL *

      0.15 pmol/ μl

      52.7 kDa

      To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a RbcL signal in this range.

      A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control.

      AS04 042S

      PsaC *

      0.15 pmol/μl

      11.5 kDa#

      To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 µl). For most applications a sample load of 0.2 µg of chlorophyll will give a PsaC signal in this range.

      A 2 ul load is optimal for most chemiluminescent detection systems as a positive control.


      *These proteins are larger than a respective native protein due to the addition of His-tag

      * For reconstitution of standards see lable on respective tube.


      Product information - Secondary antibody:

      AS09 602-trial Goat anti-Rabbit IgG (H&L), HRP conjugated, 20 µl (2x10 µl)

      Product information - ECLreagent:

      AS16 ECL-N-10 AgriseraECL Bright (10 ml trial pack)

      Educational information about Quantitative western blot can be found here:  detailed method description, video tutorial

      Additional information (application): Estimation of PSI to PSII ratio can be done using quantitative western blot technique using anti-PsaC (PSI) and PsbA (PSII) antibodies.

      References:
      Brown et al. (2007). Resource dynamics during infection of Microm
      onas pusilla by virus MpV-SP1. Environmental Microbiology 9(11): 2720-2727. Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.
    • Product Citations
    • Selected references: Abramson (2018). CARBON PARTITIONING IN ENGINEERED CYANOBACTERI UM FOR THE STUDY OF FEEDBACK INHIBITION OF PHOTOSYNTHESIS. Michigan State University, ProQuest Dissertations Publishing, 2018. 10826228.
      Morash et al. (2007) Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Can J. of Bot. 85: 476-483(8)
      Bouchard et al. (2006) UVB effects on the photosystem II-D1 protein of phytoplankton and natural phytoplankton communities. Photochem and Photobiol 82: 936-951.
      MacKenzie et al (2005). Large reallocations of carbon, nitrogen and photosynthetic reductant among phycobilisomes, photosystems and Rubisco during light acclimation in Synechococcus elongatus are constrained in cells under low environmental inorganic carbon. Arch of Microbiol. 183: 190 - 202.
Close