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Photosynthesis Tool Kit - quantitation

AS04 051set | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants, algae, cyanobacteria

This discounted antibody set contains the following high titer, well published antibodies and is suitable for Western blot using samples from a wide range of species making. This product suitable for teaching labs or mutant screening.


Photosynthesis Tool Kit - quantitation in the group Antibodies for Plant/Algal  / Global Antibodies at Agrisera AB (Antibodies for research) (AS04 051set)

DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

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The kit contains following products:

AS03 037, Rabbit anti-RbcL (Form I) (50 µl)
AS10 939, Rabbit anti-PsaC (50 µl)
AS05 084, Rabbit anti-PsbA (50 µl)
AS01 017S, Rubisco protein standard (100 µl)
AS04 042S, PsaC protein standard (100 µl)
AS01 016S, PsbA protein standard (100 µl)
AS09 602-trial, Goat anti-Rabbit IgG (H&L), HRP conjugated (2 x 10 µl)
AS16 ECL-N-10, AgriseraECL Bright (10 ml trial pack)


Product Information

Immunogen KLH-conjugated syntetic peptides for respective antibodies, see product info sheets
Host Rabbit. Secondary antibody: Goat.
Clonality Polyclonal
Quantity 50 µl of respective antibody. 100 µl of each protein standard. 2 x 10 µl of secondary antibody. 10 ml of ECL reagent.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications western blot (WB)

Reactivity

Confirmed reactivity Algae, Cyanobacteria, Higher plants

Application examples

Additional information

Additional information Product information - Primary antibodies:

Product number:

Product name:

Reconstitution:

Recommended dilution:

AS03 037

Rabbit Anti-RbcL

Global antibody

For reconstitution see lable on respective tube.

1:5000-10 000 with ECL

AS10 939

Rabbit Anti-PsaC

Global antibody

For reconstitution see lable on respective tube.

1:1000 with ECL

AS05 084

Rabbit Anti-PsbA

Global antibody

For reconstitution see lable on respective tube.

1:10 000 with ECL


* All primary antibodies in this kit are raised in rabbits.


Product information - Protein standards:

Product number:

Product name:

Concentration:

Size:

Western Blot – Positive Control:

AS01 016S

PsbA *

0.25 pmol/ μl

41.5 kDa#

To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a PsbA signal in this range.

A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control.

AS01 017S

RbcL *

0.15 pmol/ μl

52.7 kDa

To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 ul). For most applications a sample load of 0.2 ug of chlorophyll will give a RbcL signal in this range.

A 2 ul load is optimal for most chemiluminescent detection systems to use as a positive control.

AS04 042S

PsaC *

0.15 pmol/μl

11.5 kDa#

To generate a standard curve, 3 loads are suggested (0.5, 2 and 4 µl). For most applications a sample load of 0.2 µg of chlorophyll will give a PsaC signal in this range.

A 2 ul load is optimal for most chemiluminescent detection systems as a positive control.


*These proteins are larger than a respective native protein due to the addition of His-tag

* For reconstitution of standards see lable on respective tube.


Product information - Secondary antibody:

AS09 602-trial Goat anti-Rabbit IgG (H&L), HRP conjugated, 20 µl (2x10 µl)

Product information - ECLreagent:

AS16 ECL-N-10 AgriseraECL Bright (10 ml trial pack)

Educational information about Quantitative western blot can be found here:  detailed method description, video tutorial

Estimation of PSI to PSII ratio can be done using quantitative western blot technique using anti-PsaC (PSI) and PsbA (PSII) antibodies.

References:
Brown et al. (2007). Resource dynamics during infection of Microm
onas pusilla by virus MpV-SP1. Environmental Microbiology 9(11): 2720-2727. Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.

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