GDC-H | H protein of glycine decarboxylase complex (GDC)

purified GDC-H protein from Spinacia oleracea

16 kDa

Application example

15 µg of total protein from  Arabidopsis thaliana leaf extract has been loaded per lane. Primary antibody has been used in 1: 5000 dilution using chemiluminescent detection.

Courtesy of Dr Olivier Keech, UPSC, Umeå, Sweden

AS06 203A | Anti- Idh | isocytrate dehydrogenase rabbit antibodies, marker of mitochondrial matrix

AS07 212 | Anti-VDAC1 marker, rabbit antibodies for mitochondrial outer membrane

Plant protein extraction buffer

Secondary antibodies

The Glycine decarboxylase complex (GDC) is abundant in mitochondria matrix of C3 leaves and functions in photorespiratory carbon recovery. GDC  enzyme can account for up to 50% of matrix protein, and is responsible for the most prominent metabolic activity in the mitochondria of illuminated leaves, photorespiration. GDC is a multienzyme complex composed of four component enzymes, the P-, H-, T-, and L-proteins and is responsible for the conversion of glycine produced in the peroxisome to serine in the mitochondria during photorespiratory cycle. The H-protein plays a key role as a mobile substrate that commutes between the other subunits, allowing its lipoic acid “arm” to visit the active sites of the other three components.