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AGO set
Tag Antibody Set 

GDC-H | H protein of glycine decarboxylase complex (GDC)

AS05 074  |  Clonality: Polyclonal  |  Host: Rabbit  |   Reactivity: A. thaliana,P.hybrida cv. Mitchell, S. oleracea, T. aestivum, V. faba  |  cellular [compartment marker] of mitochondrial matrix

GDC-H | H protein of glycine decarboxylase complex (GDC) in the group Antibodies for Plant/Algal  / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS05 074)

PRODUCT INFORMATION IN PDF


Datasheet Product citations Protocols Customer reviews

Product Information

Immunogen

purified GDC-H protein from Spinacia oleracea

Host Rabbit
Clonality Polyclonal
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 200 µg
Reconstitution For reconstitution add 200 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5 000 (WB)
Expected | apparent MW

16 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Petunia hybrida cv. Mitchell, Spinacia oleracea, Triticum aestivum, Vicia faba
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

Application example

 

western blot detection using anti-GDC-H antibody

15 µg of total protein from  Arabidopsis thaliana leaf extract has been loaded per lane. Primary antibody has been used in 1: 5000 dilution using chemiluminescent detection.

Additional information

Additional information Cellular [compartment marker] of mitochondrial matrix
This antibody can be used on total cell extract of Arabidopsis thaliana.

Related products

Related products

AS06 203A | Anti- Idh | isocytrate dehydrogenase rabbit antibodies, marker of mitochondrial matrix

AS07 212 | Anti-VDAC1 marker, rabbit antibodies for mitochondrial outer membrane

Plant protein extraction buffer

Secondary antibodies

Background

Background

The Glycine decarboxylase complex (GDC) is abundant in mitochondria of C3 leaves and functions in photorespiratory carbon recovery. GDC  enzyme can account for up to 50% of matrix protein, and is responsible for the most prominent metabolic activity in the mitochondria of illuminated leaves, photorespiration. GDC is a multienzyme complex composed of four component enzymes, the P-, H-, T-, and L-proteins and is responsible for the conversion of glycine produced in the peroxisome to serine in the mitochondria during photorespiratory cycle. The H-protein plays a key role as a mobile substrate that commutes between the other subunits, allowing its lipoic acid “arm” to visit the active sites of the other three components.

Product citations

Selected references Lynch et al. (2017). Multifaceted plant responses to circumvent Phe hyperaccumulation by downregulation of flux through the shikimate pathway and by vacuolar Phe sequestration. Plant J. 2017 Dec;92(5):939-950. doi: 10.1111/tpj.13730. (Petunia hybrida cv. Mitchell)
Bancel et al. (2015). Proteomic Approach to Identify Nuclear Proteins in Wheat Grain. J Proteome Res. 2015 Sep 8.
Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544.
Córdoba-Cañero et al. (2011). Arabidopsis ARP endonuclease functions in a branched base excision DNA repair pathway completed by LIG1. The Plant J in print

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