Predicted reactivityPredicted reactivity can be checked based on sequence alignment between a trarget protein from a species of confirmed and validated reactivity, and the full length protein sequence from species we intend to use an antibody for. The antigens that are used for antibody development can vary from 5 to 20 amino acid long peptides, to partial or full length proteins. Therefore, comparing the overall target protein sequence conservation between species is not relevant.What to provide us with for predicted reactivity check?
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Latest
Are monoclonal antibodies better, compare to polyclonal?2023-11-30 Why, to detect some proteins, 6M urea must be included in the extraction and loading buffers?
2023-09-01 I have not used this antibody before, what conditions of Western blot do you recommend?
2023-07-27 Which subclasses of immunoglobulin G are found in rabbit serum?
2023-07-18 Antibody suddenly does not work any longer, what can be the reason?
2023-07-11 Can an antibody made to a full-length protein distinguish between 2 isoforms?
2023-05-10 What is a reason for so called "ghost bands" to appear on blots?
2023-03-30 One of the ways to remove background on a Western blot
2023-03-24 How to predict in which technique a given antibody may work?
2023-03-10 Which capture antibody to use in ELISA?
2023-03-06
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