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DYKDDDDK (binds to Sigma FLAGŪ, clone FG4R)

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AS15 2871
  | Clonality: monoclonal  |  Host: Mouse  |  Reactivity: N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins

DYKDDDDK (binds to Sigma FLAGŪ, clone FG4R) in the group Tag Antibodies / DYKDDDDK (binds to SigmaFLAGŪ) at Agrisera AB (Antibodies for research) (AS15 2871)

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Product Information

Immunogen

KLH-conjugated DYKDDDDK (FLAG) synthetic peptide

Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Protein A purified
Format Liquid in 10mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide
Quantity 50 µg
Storage

Store at -20°C for up to 2 years.

Tested applications Dot blot (Dot), ELISA (ELISA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution 1 : 1000-5000 (1-5ng) (WB) (incubate for one hour at room temperature). 1 : 500-1 :2000 (IS) For best results with other assays (Dot, ELISA, IP), please determine optimal working dilution by end user

Reactivity

Confirmed reactivity N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-FLAG antibody

20 µg of total protein from Chlamydomonas reinhardtii   Flag; 2  (1) 1xFlag 5’+ICL (1x FLAG coding sequence : GA-CTA-CAA-GGA-CGA-CGA-TGA-CAA-G) (2); 1xFlag 5’+ICL (1x FLAG codingsequence :GA-CTA-CAA-GGA-CGA-CGA-TGA-CAA-G) (3); ICL+ 3xFlag 3’end (3xFLAG codingsequence : GAC TAC AAG GAC CAC GAC GGT GAC TAC AAG GAC CAC GAC ATC GAC TAC AAG GAC GACGACGAC AAG TGA.) (4); 1xFlag 5’+ICL+ 3xFlag 3’end (5) were separated on 12 % SDS-PAGE  and blotted ON (30V) to PVDF using tank transfer. Blots were blocked with 5 % non-fat milk  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-mouse IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with BM ECL kit according to the manufacturer's instructions. Exposure time was  seconds.

Courtesy of Dr. Nadine Coosemans, Remacle lab, University of Liège, Belgium

Additional information

Additional information Working dilution for Dot Blot, ELISA and IP needs to be determined experimentally.

Antibody is purified on Protein A.

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AS15 2948
| anti-DYKDDDDK (binds to Sigma FLAG®), Europium conjugated, mouse monoclonal antibodies

AS15 2949 | anti-DYKDDDDK (binds to Sigma FLAG®), SureLight® R-Phycoerythrin conjugated,mouse monoclonal antibodies
AS15 3036 | anti-DYKDDDDK (binds to Sigma FLAG®),mouse monoclonal antibodies
AS15 3037 | anti-DYKDDDDK (binds to Sigma FLAG®), rabbit antibodies

other tag antibodies
Secondary antibodies

Background

Background FLAG-tag is a tag that can be added to a protein sequence motif DYKXXD. It can be used for affinity chromatography, to separate recombinant protein from wt proteins. It can also be used to isolate protein complexes with multiple subunits.

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Buy 2 items of this product for 214.00 €/items
Buy 3 items of this product for 195.00 €/items